华北农学报2023，Vol.38Issue(6)：211-217,7.DOI:10.7668/hbnxb.20194254
不同NDF水平开食料对羔羊生长轴及十二指肠生长相关基因表达的影响
Effect of Different NDF Levels of Starter on Growth Axis and Duodenum-Related Gene Expression in Lambs
摘要
Abstract
The aim of this trial was to investigate the effect of different levels of NDF on the expression of genes related to growth axis and duodenal growth in lambs,in order to provide a reference for the appropriate NDF level for lamb starter.Thirty-six male Hu lambs were selected for the trial and randomly divided into 15%and 25%NDF level groups.At 56 days of age,six lambs from each group were selected for slaughter,weighed for tissue and organ,and the expression of relevant genes was measured.The results showed that there was no significant effect(P>0.05)of NDF levels in the starter diet on tissue and organ mass and index;in the 15%group of lambs,hypo-thalamic growth hormone releasing hormone(GHRH),hepatic insulin-like growth factor-1(IGF-1),duodenal IGF-1 and IGF-1 receptor(IGF-1R)expressions were significantly higher in the 25%group(P<0.05);the expression of pituitary growth hormone(GH)and growth hormone receptor(GHR)in the duodenum were significantly higher in the 25%group than in the 15%group(P<0.01).The average daily gain of lambs was strongly negatively correla-ted with hypothalamic GHRH expression(P<0.01),significantly negatively correlated with hepatic GHR and duo-denal IGF-1 expression(P<0.05),and significantly positively correlated with pituitary GH and duodenal GHR ex-pression(P<0.05);pituitary GH was significantly or highly significantly negatively correlated with hepatic GHR,IGF-1,duodenal IGF-1 and IGF-1R expression(P<0.05 or P<0.01),and strongly significantly positively correla-ted with duodenal GHR expression(P<0.01);hepatic GHR was strongly significantly positively correlated with he-patic IGF-1,duodenal IGF-1 expression(P<0.01),and strongly significantly negatively correlated with duodenal GHR expression(P<0.01).In conclusion,the expression of genes related to GH-GHR levels in the growth axis of lambs is enhanced by feeding 25%NDF level open feed compared to the 15%level group.关键词
羔羊/开食料/生长轴/十二指肠/基因表达Key words
Lamb/Starter feed/Growth axis/Duodenum/Gene expression分类
农业科技引用本文复制引用
韩晓霞,赵海碧,刘国华,吕凤,庞鑫,杨帆,汪晓娟..不同NDF水平开食料对羔羊生长轴及十二指肠生长相关基因表达的影响[J].华北农学报,2023,38(6):211-217,7.基金项目
甘肃省自然科学基金项目(20JR10RA538) （20JR10RA538）
国家自然科学基金项目(31760682) （31760682）
