化工进展2023，Vol.42Issue(12)：6641-6648,8.DOI:10.16085/j.issn.1000-6613.2023-0117
苹果酸脱氢酶协同纤维素酶水解纤维素:理论分析与实验
The malate dehydrogenase synergistically hydrolyzes cellulose with cellulase:Theoretical analysis and experimental investigation
摘要
Abstract
The rigid structure of cellulose molecules is considered a serious impediment to the cellulose hydrolysis by cellulase.The disaggregation of cellulose by the addition of auxiliary proteins is an effective strategy to make the contact of cellulase with cellulose easier.Based on the documented α-glucosidase,a member of the glycoside hydrolase family 4,exhibiting synergism with the cellulase,and the analysis of protein homology as well as structure,a conjecture that the malate dehydrogenase(MDH)had a similar synergistic function was proposed.The theoretical and experimental studies on the hydrolysis of filter paper by the MDH synergism with cellulase were carried out.The interaction between MDH and crystalline cellulose was simulated by molecular docking.Theoretical analysis showed that the MDH could reduce the aggregation of cellulose by the effect of hydrogen bonds and hydrophobic interaction.The cellulose hydrolysis by the MDH synergism with cellulase,involving the addition of MDH,the amount of cellulase and the hydrolysis time were investigated.It was found that the yield of reducing sugar in the system of(50±0.50)mg filter paper,150μg MDH and 0.06FPU cellulase was approximately 3.47-fold higher than the system of cellulase alone under the condition of hydrolyzing 24h in a water bath at 50℃.An enhanced activity of 247%±28%was acquired for cellulase by the synergic effect of MDH.The scanning electron microscopy(SEM),X-ray diffraction(XRD)and Fourier transform infrared spectroscopy(FTIR)were used to analyze the filter papers before and after adding the MDH.Results showed that the MDH could destroy the crystalline regions of the cellulose,synergistically improving the efficiency of cellulose hydrolysis by cellulase.As an effective auxiliary protein,the MDH would provide an alternative for cellulose hydrolysis.关键词
辅助蛋白/苹果酸脱氢酶/纤维素酶/纤维素/水解Key words
auxiliary protein/malate dehydrogenase/cellulase/cellulose/hydrolysis分类
生物科学引用本文复制引用
董友情,唐爱星,刘幽燕,李青云..苹果酸脱氢酶协同纤维素酶水解纤维素:理论分析与实验[J].化工进展,2023,42(12):6641-6648,8.基金项目
国家自然科学基金(21276053). （21276053）
