化工进展2023,Vol.42Issue(12):6658-6665,8.DOI:10.16085/j.issn.1000-6613.2023-0203
ABR产酸-硫酸盐还原相颗粒污泥富集PHAs产生菌
Enrichment of PHAs-producing bacteria by granular sludge in ABR acidogenic sulfate-reducing phase
摘要
Abstract
In order to investigate the feasibility of synthesizing polyhydroxyalkanoates(PHAs)from sulfate organic wastewater through anaerobic treatment,a five-compartment anaerobic baffled reactor(ABR)was utilized to enrich PHAs-producing bacteria using sulfate organic wastewater as substrate,and the effects of different influent COD/SO42-ratios(12.5,9.3 and 4.0)on PHAs synthesis efficiency of granular sludge in the acidogenic sulfate-reducing phase(the first and second compartments)of ABR were examined to explore the PHA synthesis pattern in this system.The results showed that as the inflow COD/SO42-ratio decreased,the removal of COD and SO42-in the acidogenic sulfate-reduction phase shifted downstream.In the first compartment,the dominant microbial metabolism type was the butyric acid type,while in the second compartment,it shifted from the acetic acid type to the butyric acid type.The compartment with high content of PHAs in the granular sludge shifted from the first compartment to the second compartment.The best PHAs synthesis effect was observed when the inflow COD/SO42-ratio was 9.3,which led to a large enrichment of PHAs-producing bacteria and high production of PHAs in the granular sludge of the acidogenic sulfate-reduction phase.The PHAs-producing bacteria in the granular sludge were large,and the PHAs granules were densely distributed throughout the bacterial cells.In the acidogenic sulfate-reducing phase,there existed a bio-chain cooperation relationship among acid-producing bacteria(APB),fatty-acid utilized sulfate reducing bacteria(FSRB)and acetic-acid utilized sulfate reducing bacteria(ASRB),and a large amount of PHAs was formed inside the bacterial cells.This study provided theoretical support for the resource utilization of sulfate organic wastewater.关键词
聚羟基脂肪酸酯/厌氧折流板反应器/产酸-硫酸盐还原相/代谢类型/合成途径Key words
polyhydroxyalkanoates/anaerobic baffled reactor/acidogenic sulfate-reducing phase/metabolic type/synthesis pathway分类
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汪晨祥,秦永丽,蒋永荣,葛仕佳,郑国权,孙振举..ABR产酸-硫酸盐还原相颗粒污泥富集PHAs产生菌[J].化工进展,2023,42(12):6658-6665,8.基金项目
国家自然科学基金(52060004,52000046) (52060004,52000046)
广西高校中青年教师科研基础能力提升项目(2021KY0204). (2021KY0204)