河南城建学院学报2023，Vol.32Issue(6)：125-132,8.DOI:10.14140/j.cnki.hncjxb.2023.06.018
裂解多糖单加氧酶AfLPMO7的酶学特性研究
Enzymatic characterization of lytic polysaccharide monooxygenase AfLPMO7
马磊 1娄淑慧 1尹玉莹 1安姝宇 1朱超强 1韩艺 1高建民1
作者信息
- 1. 河南城建学院生命科学与工程学院,河南平顶山 467036
- 折叠
摘要
Abstract
To improve the degradation efficiency of cellulose,the lytic polysaccharide monooxygenase AfLP-MO7,derived from Aspergillus,fumigatus Z5,was expressed.The result of substrate binding experiments showed that AfLPMO7 had a strong binding ability to phosphoric acid swollen cellulose(PASC),which also could directly act on PASC and Xylan and produce reducing sugars.The saccharification experiments showed that AfLPMO7 could effectively assist the cellulase to hydrolyze the natural substrates of rice straw,wheat straw and corn straw,the maximum increases were 111.89％,38.82％and 50.95％,respectively.Kinetic experi-ments showed that AfLPMO7 had the relatively strong oxidative capacity for 2,6-DMP,the Vmax value was 109.99±3.91 U/g.Automated modeling and molecular docking suggested the zones were different for catalytic and substrate binding centers.关键词
裂解多糖单加氧酶/纤维素/还原糖/协同酶活力Key words
lytic polysaccharide monooxygenases/cellulose/reducing sugar/synergistic activity分类
农业科技引用本文复制引用
马磊,娄淑慧,尹玉莹,安姝宇,朱超强,韩艺,高建民..裂解多糖单加氧酶AfLPMO7的酶学特性研究[J].河南城建学院学报,2023,32(6):125-132,8.
