核农学报2024，Vol.38Issue(2)：256-264,9.DOI:10.11869/j.issn.1000-8551.2024.02.0256
四种类型竹种竹叶总RNA提取方法的比较研究
Comparative Analysis of Extraction Methods of Total RNA from Four Different Types of Bamboo Leaves
摘要
Abstract
To investigate the total RNA extraction method from various types bamboo,we selected the core leaves of Dendrocalamus brandisii(sympodial rhizomes with caespitose culms),Fargesia yunnanensis(sympodial rhizomes with scattered culms),Phyllostachys edulis(monopodial rhizomes),and Bashania fargesii(amphipodial rhizomes)as the experimental materials for total RNA extraction.The integrity of RNA was assessed using agarose gel electrophoresis,while the purity and concentration of RNA were determined using ultra-micro ultraviolet Photosystem.A comparison was made among the self-made TRIzol reagent,TRIzon reagent,RNA extraction kit,and improved CTAB methods regarding the quality,purity of the extracted total RNA,and the cost.We found that with self-made TRIzol reagent method,the quality of total RNA was superior and the concentration was higher but with a comparatively lower cost.With the TRIzon reagent method resulted in total RNA with slightly lower quality and concentration,albeit with a slightly higher extraction cost.However,both the RNA extraction kit and the improved CTAB method were found to be ineffective in extracting total RNA of high concentration and good quality from bamboo leaves.The findings of this study serve as a foundation for future research in the field of molecular biology pertaining to bamboo plants,and also offer valuable insights for the extraction of total RNA from other bamboo species.关键词
竹叶/RNA提取/隶属函数Key words
bamboo leaf/RNA extraction/membership function引用本文复制引用
马迎春,王曙光,詹卉,于丽霞,杨德佳,高若天,李娟..四种类型竹种竹叶总RNA提取方法的比较研究[J].核农学报,2024,38(2):256-264,9.基金项目
云南省教育厅科学研究基金项目(2023Y0783),云南省兴滇英才"青年人才专项"(XDYC-QNRC-2022-0229),"十四五"国家重点研发计划项目(2021YFD2200503-4) （2023Y0783）
