神经生长因子及其受体在高原牦牛与平原黄牛端脑中的比较分析OACSTPCD

To compare the expression and distribution of nerve growth factor and its high-affinity receptor tyrosine protein kinase A in the telencephalon of highland yaks(Bos grunniens)and plain cattles(Bos taurus),adult yak and cattle telencephalon tissues(n=5)from various altitudes were collected and characterized by immunohistochemistry(IHC),quantitative real-time PCR(qRT-PCR)and Western Blot(WB)to compare the localisation and expression levels of nerve growth factor and tyrosine protein kinase A in different regions of the telencephalon in yaks and cattles.The IHC results showed that these two factors were mainly expressed in the cytoplasm of neurons,neuroglia,and the inner wall of blood vessels in the telencephalic regions of yaks and cattles,and the intensity of their protein immunopositive reaction was higher in yaks than in cattles.The qRT-PCR results revealed that the expression of nerve growth factor gene in yaks was lower than or not different from that of cattles in the frontal cortex,cerebral white matter,and occipital cortex,and significantly higher than that of cattles in the rest of the regions(P<0.05).Tyrosine protein kinase A gene did not show significantly different expression from that of cattles only in the cerebral white matter,and significantly higher than that of cattles in the rest of the regions(P<0.05).Nerve growth factor protein had lower expression in yaks than in cattles only in the white matter of the brain,and significantly higher than in the rest of the regions(P<0.05).Tyrosine protein kinase A protein in yaks had lower expression than in cattlse or had no difference in the frontal cortex,white matter of the brain,and occipital cortex,and significantly higher than in the rest of the regions(P<0.05).The above results suggested that the up-regulation of the two factors'expression in some tissues of yaks telencephalon may be related to hypoxic stimulation,and it is speculated that the two factors synergistically exert endogenous neuroprotective effects under hypoxic conditions to maintain the normal physiological activities of animal,which provides a theoretical basis for further investigation of the roles of nerve growth factor and tyrosine protein kinase A in the hypoxic environment.