口腔疾病防治2024，Vol.32Issue(1)：22-28,7.DOI:10.12016/j.issn.2096-1456.2024.01.004
低浓度氟化钠对人牙髓细胞的成骨/成牙本质分化的影响
Effect of low concentration of sodium fluoride on osteogenic/odontogenic differentiation of human dental pulp cells
摘要
Abstract
Objective To study the effect of low concentrations of sodium fluoride on the osteogenic/odontogenic differentiation of human dental pulp cells(hDPCs)in vitro.Methods This study was reviewed and approved by the Ethics Committee.hDPCs were cultured using a modified tissue explant technique in vitro.The effects of different con-centrations of sodium fluoride on the proliferation of hDPCs were measured by methylthiazol tetrazolium(MTT)assay.Appropriate concentrations were added to the osteogenic/odontogenic differentiation induction medium,and the cells were induced in vitro.Alizarin red S staining was used to detect the osteoblastic/odontogenic differentiation ability of the cells,and the mRNA expression of the key differentiation factors was detected by RT-qPCR.Moreover,the expres-sion of key molecules of endoplasmic reticulum stress(ERS)was detected by RT-qPCR and Western blot.The data were analyzed with the SPSS 18.0 software package.Results Low concentration of NaF(0.1 mmol/L)could stimulate cell proliferation in vitro,while a high concentration(5-10 mmol/L)could inhibit cell proliferation(P＜0.05).According to the literature and the experimental data,0.1 mmol/L NaF was selected as the following experimental concentration.The levels of alizarin red S staining were increased after NaF induction of mixed osteogenic/odontogenic differentiation in vi-tro.The mRNA expression levels of key molecules for osteogenic/odontogenic differentiation,dentin sialophosphoprotein(DSPP),bone sialoprotein(BSP)and osteocalcin(OCN),were increased(P＜0.05).The mRNA levels of ERS markers(splicing x-box binding protein-1(sXBP1),glucose-regulated protein 78(GRP78)and activating transcription Factor 4(ATF4)were increased in NaF-treated cells.The protein expression levels of key ER stress molecules(phosphorylated RNA-activated protein kinase-like ER-resident kinase(p-PERK),phosphorylated eukaryotic initiation factor-2α(p-eIF2α)and ATF4)were higher in NaF-treated cells.Conclusion A low concentration of NaF promotes the osteogenic/odontogenic differentiation of hDPCs and increases the level of ER stress.关键词
人牙髓细胞/氟化钠/增殖/成骨/成牙本质分化/内质网应激/剪切X盒结合蛋白1/活化转录因子4/葡萄糖调节蛋白78/蛋白激酶样内质网激酶/真核起始因子-2α分类
医药卫生引用本文复制引用
李莉芬,韩俊力,江龙..低浓度氟化钠对人牙髓细胞的成骨/成牙本质分化的影响[J].口腔疾病防治,2024,32(1):22-28,7.基金项目
上海市青年科技英才扬帆计划(21YF1424100) （21YF1424100）
上海交通大学医学院附属第九人民医院基础研究助推计划(JYZZ183)The study was supported by the grants from Shanghai Sailing Program(No.21YF1424100) （JYZZ183）
Basic research assistance sProgram of Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medi-cine(No.JYZZ183). （No.JYZZ183）
