核农学报2024，Vol.38Issue(2)：298-307,10.DOI:10.11869/j.issn.1000-8551.2024.02.0298
赤霉素及二氢茉莉酸丙酯对抑制温州蜜柑果实浮皮的影响
Effects of Gibberellin and Propyldihydrojasmon on Inhibiting Puffing of Satsuma Mandarin Fruit
摘要
Abstract
In order to investigate the methods of preventing citrus fruit from puffing and the mechanism of gibberellin(GA3)and propyldihydrojasmon(PDJ)inhibiting peel puffing,the effects of calcium or GA3 and PDJ on the fruit puffing index and fruit quality were examined by foliar spraying on satsuma mandarin Miyagawa with delayed harvest cultivation,and the alterations in peel cell wall structure,cell wall substance content,hydrolytic enzyme activity,phytohormone content,and the relevant gene expression with GA3 and PDJ-treated were analyzed.The results demonstrated that all treatments were able to reduce the fruit puffiness index.However,only spraying twice with GA3 and PDJ-treat before harvest could control the fruit puffiness index at a low level without affecting the external and internal quality of the fruit.In the GA3-and-PDJ treatment,the activity of pectin methylesterase(PME),cellulase(Cx)and the expression of CitPME and CitCx genes was suppressed.The protopectin and hemicellulose content in the peel and fruit hardness were increased in GA3-and-PDJ treatment.Compared with congtrol,cellulose content decreasing was delayed,and the degree of membrane lipid peroxidation in the peel was reduced.Meanwhile,the expression of the CitACO gene was suppressed,which resulted in a decrease of ethylene synthesis,while the CitETR1 gene was up-regulated,and the IAA content and CitIAA27 gene expression increased.The results of this study indicated that combined spraying of GA3-and-PDJ twice was the most effective method to prevent the peel puffing of satsuma mandarin with delayed harvest cultivation.The treatment inhibited the degradation of cell wall structural substances,reducing the degree of membrane lipid peroxidation,decreasing ethylene synthesis and reducing the sensitivity of peel cells to ethylene,and improving peel IAA content,and thus inhibiting peel senescence and the occurrence of peel puffing.This research provides a theoretical and practical foundation for understanding the mechanism of citrus fruit puffing and developing strategies to control citrus puffing.关键词
宽皮柑橘/浮皮/植物激素/细胞壁代谢Key words
loose-skin citrus/puffing/phytohormone/cell wall metabolism引用本文复制引用
余文莉,余旋,刘德春,杨莉,匡柳青,宋杰,刘勇,胡威..赤霉素及二氢茉莉酸丙酯对抑制温州蜜柑果实浮皮的影响[J].核农学报,2024,38(2):298-307,10.基金项目
江西省重点研发计划"揭榜挂帅"项目(20223BBF61007),江西省自然科学基金面上项目(20224BAB205026),江西省柑橘产业技术体系项目(JXAR-07-栽培岗位) （20223BBF61007）
