核农学报2024，Vol.38Issue(2)：327-335,9.DOI:10.11869/j.issn.1000-8551.2024.02.0327
基于功能化介孔二氧化硅的贻贝中磷脂选择性纯化研究
Study on Selective Purification of Phospholipids from Mussels based on Functionalized Mesoporous Silica
摘要
Abstract
To prepare high purity marine source phospholipids,the functionalized mesoporous core-shell(KCC-1)microspheres were prepared by the microemulsion method.Furthermore,the titanium dioxide/KCC-1(TiO2/KCC-1)and graphene oxide/KCC-1(GO/KCC-1)composites were prepared by the water vapor internal hydrolysis.The morphology and structure of the prepared materials were characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM)and atomic force microscopy(AFM).The two new materials were used as the adsorbent to prepare solid phase extraction(SPE)column.The phospholipids extracted from mussels were purified by TiO2/KCC-1 SPE and GO/KCC-1 SPE,respectively.The performance of these two methods was evaluated by the enrichment factor(EF).Finally,the high purify phospholipids were successfully prepared by the superior SPE method.As a result,the microspheres of synthesized KCC-1 were 250-400 nm in diameter with good morphology,uniformed size and large specific surface area.After functionalized modification,the TiO2/KCC-1 and GO/KCC-1 composites were successfully prepared.It was found that the EFTiO2/KCC-1 was greater than EFGO/KCC-1,indicating that TiO2/KCC-1 SPE method had a superior effect on the purification of phospholipids.The phospholipid prepared by TiO2/KCC-1 SPE method had a purity of 93.57%.The results of this study could provide more technical support for the preparation of high purity marine source phospholipids.关键词
海洋源磷脂/贻贝/介孔二氧化硅/固相萃取Key words
marine source phospholipids/mussels/KCC-1/SPE引用本文复制引用
水若凡,代康慧,李倩云,王丹丽,袁婷兰,李玲,龚金炎,宋恭帅..基于功能化介孔二氧化硅的贻贝中磷脂选择性纯化研究[J].核农学报,2024,38(2):327-335,9.基金项目
国家级大学生创新创业训练计划项目(202211057027),校级大学生创新创业训练计划项目(202211057027) （202211057027）
