国际口腔医学杂志2024，Vol.51Issue(1)：52-59,8.DOI:10.7518/gjkq.2024013
长链非编码RNA小核仁RNA宿主基因22调控微小RNA-27b-3p对口腔鳞状细胞癌细胞增殖、侵袭和迁移的影响
Influences of long noncoding RNA small nucleolar RNA host gene 22 on the cell proliferation,invasion and migra-tion of oral squamous carcinoma cells by regulating microRNA-27b-3p
摘要
Abstract
Objective This study aimed to investigate the influences of long noncoding RNA small nucleolar RNA host gene(SNHG)22 on the proliferation,invasion,and migration of oral squamous cell carcinoma(OSCC)cells by regu-lating microRNA(miR)-27b-3p.Methods Cancer tissue and paracancerous tissue specimens of 52 OSCC patients were collected.Human normal oral keratinocytes(HOK)and three kinds of human OSCC cells(CAL-27,SCC-25,and HSC-3)were cultured in vitro.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of SNHG22 and miR-27b-3p in cancer tissues,adjacent tissues,HOK cells,and three kinds of OSCC cells.SCC-25 cells were transfected and separated into the following groups:control(no transfection),si-SNHG22,si-NC,miR-27b-3p inhibitor,inhibitor-NC,si-SNHG22+inhibitor-NC,and si-SNHG22+miR-27b-3p inhibitor.The proliferation of SCC-25 cells in each group was detected by cell-counting kit 8 method,and proliferation index(PI)was detected by flow cytometry.Transwell assay was applied to detect the invasion of SCC-25 cells in each group.Scratch-area healing experi-ment was applied to detect the migration of SCC-25 cells in each group.Dual-luciferase experiment was applied to verify the targeting relationship between SNHG22 and miR-27b-3p.Results Compared with adjacent tissues,SNHG22 expres-sion in OSCC cancer tissues significantly increased,and the expression of miR-27b-3p significantly decreased(P<0.05).Compared with HOK cells,SNHG22 expression significantly increased in CAL-27 cells,SCC-25 cells,and HSC-3 cells,and the expression of miR-27b-3p significantly decreased.The expression in SCC-25 cells differed the most from that in HOK cells(P<0.05).Compared with the control group,the SCC-25 cell-proliferation rate,PI,invasion number,and scratch-area healing rate in the si-SNHG22 group decreased significantly(P<0.05).The SCC-25 cell-proliferation rate,PI,invasion number,and scratch-area healing rate in the miR-27b-3p inhibitor group increased significantly(P<0.05);compared with the si-SNHG22 group.The SCC-25 cell-proliferation rate,PI,invasion number,and scratch-area healing rate in the si-SNHG22+miR-27b-3p inhibitor group increased significantly(P<0.05).Dual-luciferase experiments showed that SNHG22 had a targeting relationship with miR-27b-3p.Conclusion SNHG22 was highly expressed in OSSC,whereas miR-27b-3p was lowly expressed.SNHG22 may promote the proliferation,invasion,and migration of SCC-25 cells through sponge miR-27b-3p.The SNHG22/miR-27b-3p axis may be a new diagnostic and therapeutic target for OSCC.关键词
长链非编码RNA小核仁RNA宿主基因22/微小RNA-27b-3p/口腔鳞状细胞癌/增殖/侵袭/迁移Key words
long noncoding RNA small nucleolar RNA host gene 22/microRNA-27b-3p/oral squamous cell carci-noma/proliferation/invasion/migration分类
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周金阔,张晋弘,史晓晶,刘广顺,姜磊,刘倩峰..长链非编码RNA小核仁RNA宿主基因22调控微小RNA-27b-3p对口腔鳞状细胞癌细胞增殖、侵袭和迁移的影响[J].国际口腔医学杂志,2024,51(1):52-59,8.基金项目
河北省2022年度医科科学研究课题(20221387) Hebei Province 2022 Annual Medical Scien-ce Research Project(20221387) （20221387）
