环状RNA hsa_circ_0085576调控微小RNA-498/B细胞特异性莫洛尼鼠白血病病毒整合位点1轴对口腔鳞状细胞癌细胞迁移和侵袭的影响OA

[Abstrac]Objective This study aims to explore the effects of circular RNA hsa_circ_0085576 on cell migration and in-vasion of oral squamous cell carcinoma(OSCC)and the underlying molecular mechanism.Methods Circular RNA hsa_circ_0085576,microRNA-498(miR-498),and B-cell-specific Moloney murine leukemia virus integration site 1(BMI-1)in the cells of OSCC were detected by quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot analyses.CCK-8,scratch test,Transwell test,qRT-PCR,and Western blot were used to detect the proliferation,migra-tion,and invasion ability of SCC-15 cells and the expression of related genes and proteins.Results The expression of hsa_circ_0085576 and BMI-1 in the cells of OSCC was upregulated,and that of miR-498 was downregulated(P<0.05).The proliferative activity,scratch healing rate,number of invasive cells,and expression levels of cy-clinD1 and vimentin proteins of SCC-15 cells were downregulated,whereas the expression levels of miR-498 and E-cadherin proteins were upregulated(P<0.05).Inhibition of miR-498 expression weakened the inhibitory effect of downregulating hsa_circ_0085576 on the proliferation,migra-tion,and invasion of OSCC cells.Upregulation of BMI-1 expression attenuated the inhibitory effect of overexpressed miR-498 on the proliferation,migration,and invasion of OSCC cells.Conclusion Downregulation of hsa_circ_0085576 ex-pression inhibited the proliferation,migration,and invasion of OSCC cells by activating the miR-498/BMI-1 axis.