吉林农业大学学报2023，Vol.45Issue(6)：716-724,9.DOI:10.13327/j.jjlau.2023.0214
人参根际PGPR的分离鉴定及其促生效果
Isolation and Identification of PGPR from Ginseng Rhizosphere and Its Promoting Effect
摘要
Abstract
In order to enrich ginseng growth promoting rhizobacteria,this study used ginseng rhizo-sphere soil as materials to isolate and screen plant growth promoting rhizobacteria with probiotic po-tential by using functional medium,and isolated strains were classified and identified by morphology,physiology,biochemistry and 16S rDNA sequencing,and the effects of the screened PGPR on the growth of ginseng seedlings were verified by pot test.The results showed that 17 strains of PGPR were isolated,including 10 strains with phosphorus solubilizing ability,8 strains with iron carrier pro-ducing ability,7 strains with IAA producing ability,9 strains with nitrogen fixing effect,and 10 strains with two or more different promoting functions.They are from 8 different genera,Pseudomonas,Burk-holderia,Bacillus,Enterobacter,Agrobacterium,Paenarthrobacter,Acinetobacter,Leclercia and Pries-tia,among which the largest number of Pseudomonas accounted for 29.41％.Strain F05-44 had the strongest overall growth-promoting ability,and the pot test found that this strain promoted the growth of ginseng without fertilization,and plant height and root length increased by 38.53％and 24.65％,respec-tively,and leaf spreading period was shortened by 3-4 days,which has potential application value.关键词
人参/PGPR/分离鉴定/促生作用Key words
ginseng/plant growth promoting rhizobacteria/isolation and identification/promoting effect分类
农业科技引用本文复制引用
关雪梅,刘思雨,王义,刘思章,于靖辉,汪树理,张爱华,王艳芳..人参根际PGPR的分离鉴定及其促生效果[J].吉林农业大学学报,2023,45(6):716-724,9.基金项目
吉林省科技发展计划项目(20200403011SF,20190201171JC),吉林省教育厅科学技术项目(JJKH20170310KJ,JJKH20200362KJ),国家自然科学基金青年基金项目(31401445) （20200403011SF,20190201171JC）
