吉林农业大学学报2023，Vol.45Issue(6)：756-762,7.DOI:10.13327/j.jjlau.2021.1251
人参外部形态特征与小孢子发育时期的相关性研究
Correlation Between External Morphological Characteristics and Mi-crospore Developmental Stages of Ginseng
摘要
Abstract
By observing the external morphology and cytological characteristics of ginseng micro-spores at different developmental stages and analyzing the correlation between the developmental stages of microspores and the external morphology of plants and floral organs,this study provides a cytological basis for ginseng sexual reproduction research and microspore culture.Materials and methods Four-year-old ginseng flower buds were picked as test materials.One part of the anthers was stained and pressed into tablets to observe the changes in cytological characteristics of the micro-spore development and the other part to observe the changes in the characteristics of plants and floral organs,establishing a corresponding relationship between the developmental stages and the external morphological characteristics of the microspores.The results showed that the development of ginseng microspores has experienced tetrad stage,early mononuclear stage,middle and late mononuclear stage,vacuole stage,binuclear stage and trinuclear stage,with obvious cytological characteristics at each stage.The developmental stages of microspores are closely related to the morphological charac-teristics of plants and floral organs.When the ginseng flower bud diameter is 1.33-1.68 mm,the an-ther diameter is 0.48-0.69 mm,the peduncle length is 12.8-17.2 mm,the pedicel length is 1.34-9.57 mm,the inflorescence diameter is 1.51-1.86 cm,the petiole length is 21.6-26.6 mm,the plant height is 24-48 cm,part of the leaves are spread out,the whole flower bud is dark green,and the top area of the petals can be white,and when the anther is full,most of the microspores are at the middle and late stages of mononuclear.Conclusion The external morphological characteristics and indica-tors of plants and floral organs can serve as the basis for determining the developmental stages of mi-crospores,and the microspores at the required stage of experimental research can be easily found.关键词
人参/小孢子/发育时期/植株形态/花器形态Key words
ginseng/microspore/developmental stage/plant morphology/floral morphology分类
农业科技引用本文复制引用
王轶晗,张浩,杨鹤,杨玉坤,方平,王英平..人参外部形态特征与小孢子发育时期的相关性研究[J].吉林农业大学学报,2023,45(6):756-762,7.基金项目
吉林省科技发展计划项目(20200802015GH),吉林省科技发展计划人参产业战略提升重大科技专项(20200504001YY) （20200802015GH）
