吉林中医药2023，Vol.43Issue(12)：1459-1464,6.DOI:10.13463/j.cnki.jlzyy.2023.12.022
藤黄健骨丸调控BMP-2/Smad信号通路治疗绝经后骨质疏松症
Effect of Tenghuang Jiangu Wan in the treatment of postmenopausal osteoporosis model rats by regulating BMP-2/Smad signaling pathway
摘要
Abstract
Objective To observe the therapeutic effect of Tenghuang Jiangu Wan(THJGW)in the treatment of postmenopausal osteoporosis model rats.Methods Seventy-two female rats were randomly divided into six groups,namely,the sham surgery group(SHAM),the model group(OVX),the Xianling Gubao capsules group(XLGB),the THJGW low-dose group(THJGW-L),the THJGW medium-dose group(THJGW-M)and the THJGW high-dose group(THJGW-H).Four drug-administration groups were given the corresponding dosage of drugs by gavage at the end of 3 days of surgical modeling except that the SHAM group and the OVX group were given distilled water by gavage.After 8 weeks of continuous administration of drugs by gavage,their body mass,uterine and renal organ weight,bone strength values were measured,and their pathological changes of femur were observed,respectively at each stage after surgery.The Western-blot method was used to detect the protein expression levels of Runx2,BMP2 and Smadl in the femur of rats.Results The experimental results showed that THJGW could increase the uterine and renal organ indexes,improve the load of femur,improve the bone microstructure,increase the calcium ion deposition and increase the number of osteoblasts in OVX rats.The Western-blot results showed that the relative protein levels of Runx2,BMP-2 and Smadl in the bone tissue of OVX rats were reduced,while after THJGW treatment,their relative protein levels of Runx2,BMP-2 and Smadl were significantly increased.Conclusion THJGW can increase bone strength and improve bone tissue morphology in OVX rats,and its mechanism of action may be related to the regulation of BMP-2/Smad signaling pathway to promote bone formation,which is effective in the treatment of postmenopausal osteoporosis.关键词
藤黄健骨丸/绝经后骨质疏松症/药效/BMP-2/Smad信号通路Key words
Tenghuang Jiangu Wan/postmenopausal osteoporosis/effect of drug/BMP-2/Smad signaling pathway分类
医药卫生引用本文复制引用
张文静,王雨辰,段瑶,顾晶业,律广富,皮子凤,林喆..藤黄健骨丸调控BMP-2/Smad信号通路治疗绝经后骨质疏松症[J].吉林中医药,2023,43(12):1459-1464,6.基金项目
吉林省科技发展计划项目(20210401064YY) （20210401064YY）
