吉林中医药2023，Vol.43Issue(12)：1469-1474,6.DOI:10.13463/j.cnki.jlzyy.2023.12.024
高效液相色谱-高分辨质谱法快速筛查动物源性药食同源产品中32种抗生素兽药残留
Rapid screening of 32 kinds of veterinary drug residues in animal-derived medicine and food homologous products by high performance liquid chromatography-high resolution mass spectrometry
摘要
Abstract
Objective To establish a rapid pre-treatment and instrument screening method for 32 kinds of antibiotic residues in animal-derived medicine and food homologous products(pilose antler,semifluid extract of deer foetus and honey)based on the high performance liquid chromatography-high resolution mass spectrometry(HPLC-HRMS).Methods The samples were extracted by acetonitrile,purified by PRiME HLB solid phase extraction column,and qualitatively screened by HPLC-HRMS database(compound name,molecular formula,molecular weight,retention time,fragment ions,etc.).Results This method could quickly and accurately screen 32 kinds of antibiotic residues in animal-derived medicine and food homologous characteristic products(deer antler,deer fetal paste and honey)without standard products,with the limit of detection of 0.01 mg/kg.Conclusion This method is simple,rapid,accurate and suitable for the screening of veterinary drug residues of antibiotics in large quantities of samples,which can provide reliable technical support for the supervision of antibiotics veterinary drug residues in animal-derived medicine and food homologous products.关键词
高效液相色谱-高分辨质谱法/动物源性食品/药食同源/抗生素类兽药残留/快速筛查/数据库Key words
high performance liquid chromatography-high resolution mass spectrometry/animal-derived food/medicine and food homology/veterinary drug residues of antibiotics/rapid screening/database分类
医药卫生引用本文复制引用
周雪莼,胡婷婷,王佳慧,白静,杨颖,侯宇,张哲,张勋..高效液相色谱-高分辨质谱法快速筛查动物源性药食同源产品中32种抗生素兽药残留[J].吉林中医药,2023,43(12):1469-1474,6.基金项目
科技部国家重点研发计划项目(2019YFC1710704) （2019YFC1710704）
吉林省自然科学基金项目(YDZJ202301ZYTS204) （YDZJ202301ZYTS204）
海关总署科技项目(2020HK182) （2020HK182）
