六盘水师范学院学报2023，Vol.35Issue(6)：112-120,9.DOI:10.16595/j.1671-055X.2023.06.012
凤冈锌硒茶茶多酚提取工艺优化及其体外活性研究
Optimization of Extraction Process of Polyphenols from Fenggang Zinc Selenium Tea and Its Bioactivity in Vitro
摘要
Abstract
The polyphenols were extracted from Fenggang zinc-selenium tea using the ultrasonic-assisted method,and the yield was used as an indicator to discuss the optimal extraction process parameters.The response surface method determined opti-mal extraction conditions of Fenggang zinc selenium tea based on three single factor experiments of extraction time,materi-al-to-liquid ratio,and ethanol volume fraction.The optimal conditions obtained showed that when the volume fraction of ethanol was 59%,the extraction time was 54 min and the ratio of solid to liquid was 1:91 g/mL,the yield of tea polyphenols was the high-est,reaching 14.88%,which was close to the predicted value of 14.79%.The IC50 values of the DPPH scavenging rate and hydrox-yl radical scavenging rate were 0.87 mg/mL and 0.45 mg/mL,respectively;after dialysis for 20min minutes,the delayed absorption capacity of glucose reached 46.50%;the binding capacity with sodium bezoar cholate and sodium glycocholate reached 16.38%and 27.69%,respectively.The results showed good antioxidant activity,delayed glucose absorption,and cholate binding ability.This study provides a technical reference for further developing and utilizing zinc selenium tea.关键词
凤冈锌硒茶/茶多酚/提取工艺/体外活性Key words
Fenggang zinc-selenium tea/tea polyphenols/extraction process/activity in vitro分类
轻工纺织引用本文复制引用
王绪英,朱淼,李欣燃..凤冈锌硒茶茶多酚提取工艺优化及其体外活性研究[J].六盘水师范学院学报,2023,35(6):112-120,9.基金项目
贵州省教育厅高校青年科技人才成长项目"刺梨花青素提取及其抗氧化性和稳定性研究"(黔教合KY字[2022]060) （黔教合KY字[2022]060）
六盘水师范学院大学生科研训练项目"锌硒茶茶多酚的提取及其抗氧化、抗菌作用研究"(LPSSYDXS2184) （LPSSYDXS2184）
六盘水师范学院自然科学一般项目"刺梨多糖及黄酮降糖降脂活性成分研究"(LPSSYYBZK202210). （LPSSYYBZK202210）
