聊城大学学报（自然科学版）2024，Vol.37Issue(1)：85-91,7.DOI:10.19728/j.issn1672-6634.2023060008
益母草注射液通过其生物碱成分上调Tie-2、VEGFR2和VEGF促进斑马鱼血管新生
Motherwort Injection Promotes Angiogenesis Via Alkaloid-Induced Upregulation of Tie-2,VEGFR2 and VEGF in Zebrafish Modle
摘要
Abstract
Objective:To study the angiogenic effects of motherwort injection in a zebrafish vascular injury model,and to clarify the potential material basis and molecular mechanism.Methods:Using HPLC-PAD and TLC techniques to detect the main components of motherwort injection.Using sunitinib induced trans-genic Tg(flk 1:eGFP)zebrafish vascular injury model,after intervention with motherwort injection,the length of intersegmental blood vessels(ISVs)of zebrafish was measured,and the expression level of angio-genesis-related genes was detected by QT-PCR.Results:It was found that the main components of Leonu-rus heterophyllus injection were stachydrine,choline and trigonelline.Among them,motherwort injection,trigonelline and choline can alleviate chemically induced zebrafish vascular damage,and its mechanism in-volves that motherwort injection upregulates the expressions of Tie-2,VEGF and VEGFR2,and its main component trigonelline up-regulates the expressions of Tie-2 and VEGF,and choline up-regulates the ex-pressions of VEGF and VEGFR2.Conclusion:Motherwort injection and its main alkaloid components can promote angiogenesis by up-regulating Tie-2,VEGFR2 and VEGF.关键词
益母草注射液/血管生成/葫芦巴碱/胆碱/Tie-2/VEGFR2/VEGFKey words
motherwort injection/angiogenesis/trigonelline/choline/Tie-2/VEGF/VEGFR2分类
生物科学引用本文复制引用
李玉芝,宋肖敏婷,王雯雯,周欣雨,黎娅琳,任吉霞,曹治兴..益母草注射液通过其生物碱成分上调Tie-2、VEGFR2和VEGF促进斑马鱼血管新生[J].聊城大学学报（自然科学版）,2024,37(1):85-91,7.基金项目
国家自然科学基金项目(82174193,82374245)资助 （82174193,82374245）
