临床误诊误治2023，Vol.36Issue(7)：32-37,6.DOI:10.3969/j.issn.1002-3429.2023.07.009
PM2.5对哮喘小鼠气道重塑的作用机制研究
Mechanism of PM2.5 on Airway Remodeling in Asthmatic Mice
摘要
Abstract
Objective To investigate the effect of PM2.5 on airway remodeling induced by ovalbumin(OVA)in asth-matic mice,and to analyze its underlying mechanism.Methods In total,36 SPF female BALB/c mice were randomly divid-ed into control group,asthma group and asthma +PM2.5 group,with 12 mice in each group.Mice in the asthma group and the asthma +PM2.5 group were sensitized by intrabitoneal injection of OVA on days 1,7 and 14,and were stimulated by 1%OVA atomization on days 21-27(30 min each time for a total of 7 times).Mice in asthma +PM2.5 group were given 100 μg PM2.5 suspension nasal drops at 30 min before each OVA stimulation.The control group was treated with normal saline.Airway re-sistance was detected at 24 h after the last stimulation of mice in the three groups.Bronchoalveolar lavage fluid(BALF)and lung tissue samples were collected after the animals were sacrificed.Hematoxylin-eosin(HE)staining,PAS staining and Masson staining were used to evaluate the pulmonary inflammation,trophy cell hyperplasia and collagen fiber deposition.The expression of α-smooth muscle actin(α-SMA)in lung tissue was detected by immunohistochemistry,and the levels of inter-leukin-4(IL-4)and interleukin-5(IL-5)in BALF were determined by ELISA.The expression level of p38 mitogen-activated protein kinase(p38 MAPK)/nuclear factor-κB(NF-κB)signaling pathway related proteins in mouse lung tissues was detec-ted by Western blot.Results Compared with the asthma group,the airway resistance and inflammation scores of asthma + PM2.5 group were increased,Goblet cells were increased,the collagen fiber hyperplasia was obvious,and the levels of IL-4 and IL-5 in BALF were also significantly increased(P＜0.05).Compared with the asthma group,the protein expression lev-els of transforming growth factor-β(1.25±0.17 vs.0.87±0.25),p-p38 MAPK/p38 MAPK(1.24±0.24 vs.0.74±0.19),p-NF-κB p65/NF-κB p65(1.02±0.14 vs.0.70±0.10)in lung tissue of asthma + PM2.5 group were increased(P＜0.05).Conclusion PM2.5 can aggravate airway remodeling in asthmatic mice by activating the p38 MAPK/NF-κB sig-naling pathway.关键词
PM2.5/哮喘/气道重塑/p38丝裂原活化蛋白激酶类/核因子-κB/杯状细胞/白细胞介素-4/小鼠,BALB/cKey words
PM2.5/Asthma/Airway remodeling/p38 mitogen-activated protein kinases/NF-κB/Goblet cells/In-terleukin-4/Mice,BALB/c分类
医药卫生引用本文复制引用
李章紊,高洁,刘健玲,王爱利,李中鹏,吴健..PM2.5对哮喘小鼠气道重塑的作用机制研究[J].临床误诊误治,2023,36(7):32-37,6.基金项目
国家自然科学基金资助项目(面上项目)(81970012) （面上项目）
