临床误诊误治2023，Vol.36Issue(7)：38-43,6.DOI:10.3969/j.issn.1002-3429.2023.07.010
漆黄素对去卵巢大鼠骨质疏松症的影响
Effects of Fisetin on Osteoporosis in Rats Undergoing Ovariectomy
摘要
Abstract
Objective To investigate the effects of Fisetin on osteoporosis in rats undergoing ovariectomy.Methods A total of 50 female SD rats were treated with bilateral ovariectomy to construct a model of osteoporosis,and divided into model group,Fisetin 10 mg/kg group,Fisetin 20 mg/kg group,Fisetin 40 mg/kg group and positive control group,with 10 rats in each group.Fisetin group was given different concentrations of Fisetin intervention,and the positive control group was given estradiol(E2)for 8 consecutive weeks.Another 10 rats were selected as sham operation group.Serum alkaline phos-phatase(ALP),osteocalcin(OCN),E2,carboxyl C-terminal cross-linked peptide of type Ⅰ collagen(CTX-Ⅰ),bone γ-carboxyl glutamate protein(BGP)and bone mineral density(BMD)were detected in each group.Bone morphology was ob-served by HE staining.Bone indexes[bone volume fraction(BV/TV),trabecular thickness(Tb.Th),trabecular separation(Tb.Sp),trabecular number(Tb.N)]were detected by micro-CT,and bone biomechanical parameters(femoral neck load-ing force,strength of tibial axis,lumbar compression force)were detected by Micro-CT.The expression levels of Runt-associ-ated transcription factor 2(Runx2),low-density lipoprotein receptor-associated protein-5(Lrp-5),β-catenin and osteoblast-specific transcription factor Osterix(Osx)were detected by Western blot.Results Compared with sham operation group,ALP,OCN,Tb.Sp and CTX-Ⅰ in model group were significantly increased.The levels of E2,BMD,BV/TV,Tb.Th,Tb.N,BGP,femoral neck loading force,strength of tibial axis,lumbar compression force,Runx2,Lrp-5,β-catenin and Osx were significantly decreased(P＜0.05).HE staining showed loose arrangement of trabeculae,destruction of reticular struc-ture,reduction of trabecular number and increase of fracture points.Compared with model group,ALP,OCN,Tb.Sp and CTX-Ⅰin Fisetin 40 mg/kg group and positive control group were significantly decreased.The levels of E2,BMD,BV/TV,Tb.Th,Tb.N,BGP,femoral neck loading force,strength of tibial axis,lumbar compression force,Runx2,Lrp-5,β-catenin and Osx were significantly increased(P＜0.05).HE staining showed that the trabecular arrangement and reticular structure of the femoral bone were obviously restored,and there were higher bone connections.Conclusion Fisetin can improve bone stress in osteoporosis rats after ovariectomy,and its mechanism of action is related to promoting Wnt/β-catenin pathways and reducing rate of high bone turnover.关键词
骨质疏松,绝经后/漆黄素/骨钙素/骨密度/骨体积分数/股骨颈加载力/成骨细胞特异性转录因子/大鼠,Sprague-DawleyKey words
Osteoporosis,postmenopausal/Fisetin/Osteocalcin/Bone mineral density/Bone volume fraction/Fem-oral neck loading force/Osteoblast-specific transcription factor/Rats,Sprague-Dawley分类
医药卫生引用本文复制引用
杨鑫,李源力,蒋萍,王冶,敬雪明..漆黄素对去卵巢大鼠骨质疏松症的影响[J].临床误诊误治,2023,36(7):38-43,6.基金项目
四川省教育厅课题(17ZB0174) （17ZB0174）
