棉花学报2023，Vol.35Issue(5)：353-364,12.DOI:10.11963/cs20220049
缩节胺和硝普钠对棉花幼苗根际土壤酶活性及细菌群落的影响
Foliar application of 1,1-dimethyl piperidinium chloride and sodium nitroprusside effects on soil enzyme activities and rhizosphere bacterial community of cotton seedlings
摘要
Abstract
[Objective]The study aims to examine the rhizosphere soil enzyme activity and bacterial community response to foliar application of 1,1-dimethyl-piperidinium chloride(DPC)and sodium nitroprusside(SNP)at the cotton seedling stage,and to screen rhizoshpere events potentially involved in cotton seedling growth.[Method]An upland cotton line A201 was grown in nursery trays under a controlled environment.The treatment with DPC of 50 mg·L-1 and SNP of 500 μmol·L1,respectively,was foliar-applied at the one-leaf-one-heart stage,and the treatment with deionized water application was selected as the control.The rhizosphere soil was sampled to quantify the activities of sucrase,urease,catalase,and alkaline phosphate at the three-leaf-one-heart stage.The rhizosphere bacteria composition examined by 16S rRNA sequence analysis.[Result]The root growth,stem diameter,and dry mass of whole cotton plant were significantly increased by the DPC and SNP treatments.The activities of urease and sucrase were significantly improved by the DPC treatment.SNP application significantly enhanced sucrase activity but significantly decreased urease activity,while DPC treatment and SNP treatment had no significant effect on the activities of catalase and alkaline phosphatase.DPC application increased the relative abundance of Patescibacteria and Simpson's diversity index of soil bacterial community,but reduced the relative abundances of Chloroflexi and Acidobacteriota and Shannon Wiener's diversity index of soil bacterial community.The results of redundancy analysis on family and genus level showed that the abundances of Saccharimonadaceae and TM7a were higher in the DPC treatment;the abundance of Cellvibrionaceae was greater in the SNP treatment;the abundance of Rhizobiaceae was greater in the DPC and SNP treatments.The control exhibited greater Nitrospiraceae and Nitrospira abundances compared with the DPC and SNP treatments.Urease activity was positively correlated with Saccharimonadaceae and TM7a relative abundances.[Conclusion]DPC and SNP could promote the growth of cotton roots and aerial parts,and influence the soil enzyme activity and bacterial community composition in the rhizosphere.关键词
棉花/缩节胺/硝普钠/土壤酶/细菌群落/丰度/多样性Key words
cotton/mepiquate chloride/sodium nitroprusside/soil enzyme/bacteria community/abundance/diversity引用本文复制引用
吴建飞,刘茜,李璇,温天旺,杨文亭,汤飞宇..缩节胺和硝普钠对棉花幼苗根际土壤酶活性及细菌群落的影响[J].棉花学报,2023,35(5):353-364,12.基金项目
国家自然科学基金(31960385,31560364) （31960385,31560364）
