昆明医科大学学报2023，Vol.44Issue(12)：6-12,7.DOI:10.12259/j.issn.2095-610X.S20231201
正中神经电刺激对缺血性脑卒中大鼠突触可塑性的影响
Effect of Median Nerve Electrical Stimulation on Synaptic Plasticity in Ischemic Stroke Rats
摘要
Abstract
Objective To investigate the effects and mechanisms of median nerve electrical stimulation on synaptic plasticity in ischemic stroke rats.Methods 18 healthy male SD rats were randomly divided into Sham group(n = 6),ischemic stroke group(MCAO group,n = 6)and median nerve electrical stimulation group(MNES group,n = 6).The left middle cerebral artery occlusion model of rats was established by thread plug method.Thread plug was not inserted in sham group.The median nerve electrical stimulation group was given median nerve electrical stimulation intervention on the 3rd day after modeling,and intervention on the next day.After intervention for 7 times,behavioral detection,HE staining was used to detect median nerve injury.Nissl staining was used to detect cerebral infarction volume.Western blot was used for detection of the expression level of proteins related to synaptic plasticity,and electron microscopy was performed.Results HE staining showed that median nerve electrical stimulation did not cause damage to the median nerve in stroke rats,and the median nerve membrane was intact without obvious inflammatory cells.Compared with MCAO group,the neural function,motor function and coordination of the injured forelimb in MNES group were significantly improved(P<0.01).Compared with MCAO group,cerebral infarction volume in MNES group was significantly reduced(P<0.05),the pyknosis of Nissl bodies in ischemic penumbra decreased.Compared with MCAO group,the expression levels of synaptic plastication-related proteins PSD95 and synI in the cortex of MNES group were significantly up-regulated after median nerve electrical stimulation(P<0.05),the number of synapses in the ischemic cortex increased significantly(P<0.01).Conclusion Median nerve electrical stimulation is a safe and effective therapeutic measure to improve nerve function after stroke,and its mechanism is related to promoting synaptic plasticity.关键词
正中神经电刺激/缺血性脑卒中/突触可塑性Key words
Median nerve electrical stimulation/Ischemic stroke/Synaptic plasticity分类
医药卫生引用本文复制引用
张成才,宁蓉,陈娜,彭艺晨,周丽,杨晰宸,卢静怡,张彭跃,李蕊..正中神经电刺激对缺血性脑卒中大鼠突触可塑性的影响[J].昆明医科大学学报,2023,44(12):6-12,7.基金项目
国家自然科学基金资助项目(81960731) （81960731）
云南省教育厅科学研究基金资助项目(2023Y0434) （2023Y0434）
云南省地方高校联合专项-面上基金资助项目(202001AO070004) （202001AO070004）
