临床输血与检验2023，Vol.25Issue(6)：727-732,6.DOI:10.3969/j.issn.1671-2587.2023.06.002
安钠咖对人脐静脉血管内皮细胞损伤的研究
Study on Caffeine Sodium Benzoate-induced Injury of Human Umbilical Vein Endothelial Cells
摘要
Abstract
Objective Long-term use of large-dose caffeine sodium benzoate,a psychotropic drug,has a great impact on the physical and mental health of users,leading to not only addiction but also varieties of diseases.This study was to investigate the mechanism of endothelial cell dysfunction mediated by caffe natrium causing coagulation dysfunction.Methods Based on clinical data,the effects of sodium ampoule on coagulation function and related diseases were examined.β-galactosidase staining was done to observe the stimulation of human umbilical vein endothelial cells(HUVECs)using 25%sodium cafe patient serum +75%normal human serum,50%sodium cafe patient serum +50%normal human serum,75%sodium cafe patient serum +25%normal human serum,sodium cafe patient serum and lipopolysaccharide,and the effects of sodium ampoule serum on HUVECs were analyzed via the HUVEC cell tube experiment to test the ability of cells to form blood vessels.Results Long-term consumption of caffeine sodium benzoate seriously affected the coagulation function of patients,and the proportion of positive cells increased with higher coffee with sodium ampna serum(P<0.05),i.e.senescent cells were elevated,and cell tube experiments showed that HUVECs blood vessel formation rate decreased after serum stimulation in patients with caffeine sodium benzoate(P<0.05).Conclusion Long-term consumption of caffeine sodium benzoate can lead to abnormal function of vascular endothelial cells in patients and hence coagulation dysfunction.关键词
安钠咖/血管内皮细胞/凝血障碍Key words
Caffeine sodium benzoate/Vascular endothelial cell/Coagulation disorders分类
医药卫生引用本文复制引用
郭蓉,苏日古嘎,李玮馨,卫家乐,于天为..安钠咖对人脐静脉血管内皮细胞损伤的研究[J].临床输血与检验,2023,25(6):727-732,6.基金项目
本课题受内蒙古自然科学基金(No.2022MS08053)、内蒙古自治区卫健委卫生科技规划项目(No.202201360)、内蒙古自治区科技计划项目(No.2021GG0143)、内蒙古自治区科技计划项目(No.2022YFSH0031)资助 （No.2022MS08053）
