昆明医科大学学报2023，Vol.44Issue(12)：20-24,5.DOI:10.12259/j.issn.2095-610X.S20231203
Mertk介导NF-κb通路影响雪旺细胞炎症反应的作用
Role of Mertk-mediated NF-κb Pathway in Inflammatory Response of Schwann Cells
摘要
Abstract
Objective To explore the regulatory effect of Mertk expression level on NF-κb pathway in rat Schwann cells and its possible mechanism.Methods Rat Schwann cells were cultured in vitro,and the expression of Mertk in Schwann cells exposed to high glucose was detected by Western blot.Co-immunoprecipitation was used to detect the interaction between endogenous Mertk and Ikbkb.Western blot was used to detect the expression levels of Ikbkb,P65 and tumor necrosis factor-α in Schwann cells after Mertk silencing.The protein expressions of Mertk,Ikbkb and P65 after silencing Mertk were detected by immunofluorescence.Results Mertk was expressed in Schwann cells,and the expression level increased with the increase of glucose concentration.Co-immunoprecipitation assay showed that Mertk interacted with Ikbkb in rat Schwann cells.Compared with the control group,the expression level of Mertk was significantly decreased(P<0.05),while Ikbkb,P65 and TNF-α were significantly increased(P<0.05)after knock down expression of Mertk in Schwann cells.Immunofluorescence experiments showed that the fluorescence of Mertk was decreased,and the fluorescence of Ikbkb and P65 was increased in the silenced Schwann cells.Conclusion After the expression of Mertk is decreased,it can mediate the regulation of NF-κb pathway in Schwann cells through interaction with Ikbkb,and up-regulate the expression of P65 and inflammatory factor TNF-α.关键词
Mertk/Ikbkb/核因子κB/雪旺氏细胞/糖尿病周围神经病变Key words
Mertk/Ikbkb/Nuclear factor kappa-b/Schwann cells/Diabetes peripheral neuropathy分类
医药卫生引用本文复制引用
付怡丹,陈文婷,苏晓杨,赵燕,兰丹凤,杨秋萍..Mertk介导NF-κb通路影响雪旺细胞炎症反应的作用[J].昆明医科大学学报,2023,44(12):20-24,5.基金项目
云南省科技厅科技计划基金资助项目(202001AY070001-159) （202001AY070001-159）
