内蒙古民族大学学报（自然科学版）2023，Vol.38Issue(6)：519-523,5.DOI:10.14045/j.cnki.15-1220.2023.06.007
蓖麻叶总黄酮纯化工艺优化及抗氧化研究
Study on Purification and Antioxidant Activity of Total Flavonoids from Castor Leaves
摘要
Abstract
Abstrac:The purification conditions and antioxidant activity of total flavonoids from castor leaves were studied in this paper.By comparing the adsorption and desorption capacity of six macroporous resins,HPD100 was final-ly determined to be the best.The results showed that the optimal purification conditions were as follows:the con-centration of loading sample was 0.98 mg·mL-1,and the flow rate was 2 BV·h-1,pH 4,and the extract concentra-tion was 70%,and the elution flow rate was 3 BV·h-1.The purity of total flavonoids from castor leaves reached 56.34%.Total flavonoids at 1.0 mg·mL-1 demonstrated DPPH scavenging rate of 78.51%.It is concluded that HPD100 macroporous resin is suitable for the purification of total flavonoids from castor leaves and the total fla-vonoids have antioxidant activity.关键词
蓖麻/总黄酮/大孔树脂Key words
Castor/Total flavonoids/Macroporous resin分类
农业科学引用本文复制引用
穆莎茉莉,袁晓霞,李国瑞,包英才..蓖麻叶总黄酮纯化工艺优化及抗氧化研究[J].内蒙古民族大学学报（自然科学版）,2023,38(6):519-523,5.基金项目
内蒙古自治区高校蓖麻产业工程技术研究中心内蒙古自治区蓖麻育种重点实验室开放基金项目(MDK201733) （MDK201733）
