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江苏省生物医学实验室科研人员暴露调查分析OACSTPCD

Investigation on exposure of scientific researchers in biomedical laboratories of Jiangsu Province

中文摘要英文摘要

目的:检测江苏省生物医学实验室科研人员体内环境污染物残留状况,为实验室生物安全制度的制定提供依据.方法:在江苏省南京、苏州、盐城、淮安4个地区高校内分别选取100例生物医学实验室科研人员和100例非科研人员为研究对象,采用问卷调查了解不同人群接触环境污染物的现状,并进一步收集研究对象的晨尿样本,其中科研人员和非科研人员分别收集到了95例和94例样本.采用β-葡萄糖醛酸苷酶水解法和高效液相色谱-串联质谱法检测尿液中邻苯二甲酸盐[di(2-ethylhexyl)phthalate,DEHP]、双酚A和苯并芘的含量.结果:江苏省内生物医学实验室科研人员尿液中DEHP、双酚A、苯并芘的残留量[(1 359.0±251.0)μg/g、(284.4±134.0)μg/g、(109.0±43.2)ng/g)]显著高于对照组人员[(489.7±103.1)μg/g、(177.3±86.4)μg/g、(80.2±31.5)ng/g,P<0.001].其中苯并芘在南京和苏州地区实验室人员体内含量较高[(132.4±37.1)ng/g、(139.3±32.8)ng/g],DEHP在各地区实验室人员体内没有明显差异,而盐城地区实验室人员体内的双酚A含量最高[(386.1±202.1)μg/g].结论:江苏省各地区生物医学实验室科研人员体内积累的环境污染物普遍高于非实验室人员,这可能与日常实验室工作相关,因此实验室科研人员更应注重防护.

Objective:To detect the residual levels of environmental pollutants in the bodies of biomedical laboratory researchers in Jiangsu Province and provide a basis for the establishment of laboratory biosafety regulations.Methods:A total of 100 biomedical laboratory researchers and 100 non-researchers were selected as subjects from universities in four regions of Jiangsu Province:Nanjing,Huai'an,Yancheng,and Suzhou.A questionnaire survey was conducted to understand the current exposure to environmental pollutants among different groups.Morning urine samples were collected from the participants,with 95 samples from researchers and 94 samples from non-researchers.The urinary levels of di(2-ethylhexyl)phthalate(DEHP),bisphenol A(BPA),and benzo[a]pyrene were measured using β-glucuronidase hydrolysis and high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Results:The levels of DEHP,BPA,and benzo[a]pyrene in the urine of biomedical laboratory researchers in Jiangsu Province[(1 359.0±251.0)μg/g,(284.4±134.0)μg/g,(109.0±43.2)ng/g]were significantly higher than those in the control group's urine[(489.7±103.1)μg/g,(177.3±86.4)μg/g,(80.2±31.5)ng/g,P<0.001].Benzo[a]pyrene levels were higher in the urine of laboratory researchers in Nanjing and Suzhou[(132.4±37.1)ng/g,(139.3±32.8)ng/g],while DEHP showed no significant differences among laboratory researchers in different regions.However,the levels of BPA in laboratory researchers from Yancheng were the highest[(386.1±202.1)μg/g].Conclusion:Biomedical laboratory researchers in various regions of Jiangsu Province have higher accumulations of environmental pollutants in their bodies compared to non-laboratory personnel.This could be associated with their daily laboratory work,emphasizing the need for laboratory researchers to pay more attention to protecting themselves from environmental pollutants.

张燕;黄剑;刘红;唐梅;张芙蓉;吴菁;沈宁;薛斌

南京医科大学附属逸夫医院医学工程部,江苏 南京 211112中国药科大学生命科学与技术学院,江苏 南京 211198南京医科大学附属逸夫医院中心实验室,江苏 南京 211112瑞测精准医学检测(上海)有限公司,上海 200120南京医科大学附属逸夫医院中心实验室,江苏 南京 211112||南京医科大学附属逸夫医院检验科,江苏 南京 211112

预防医学

生物医学实验室科研人员环境污染物尿液

biomedical laboratoryscientific researcherenvironmental pollutanturine

《南京医科大学学报(自然科学版)》 2024 (001)

65-71 / 7

国家自然科学基金面上项目(32271187,32071142);江苏省科学技术协会调研课题(JSKXKT2020013);江苏高校"青蓝工程"中青年学术带头人(KY520R202025);南京市江宁区科技惠民计划项目(2023003S)

10.7655/NYDXBNS0N230404

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