农业科学研究2023，Vol.44Issue(4)：26-33,8.
灵武长枣叶阿拉伯半乳糖蛋白分布及光合特性
Distribution of Arabinogalactan Proteins and Photosynthesis Characteristics in Ziziphus jujuba Mill'Lingwuchangzao'Leaf
摘要
Abstract
To reveal the distribution of arabinogalactan proteins AGPs and photosynthesis characteristics and drought adaptation characteristics in different development periods leaf of Ziziphus jujuba Mill'Lingwuchangzao',leaves of four periods were used as experimental material to study the distribution of AGPs by methods of immunofluorescence localization,and the photosynthesis characteristics and the soluble suger contents index of leaf were determined.The results showed that:① the external tangential cell walls of leaf epidermis cells in different developmental stages formed quite thick cuticles,antigen fluorescence of AGPs recognized by antibody distributed more copious.The mesophyll consisted entirely of developed palisade tissue cells,the cell walls and cell interiors of the mesophyll distributed antigen fluorescence of AGPs recognized by antibody in different periods leaf,the mesophyll was the main tissue of leaf AGPs distribution and the later distribution was enhanced.The cell walls and cell interiors of xylem and phloem of vascular bundles in main veins,lateral veins and veinlets located in the mesophyll distributed mass antigen fluorescence of AGPs recognized by antibody in different development periods,there were no antigen fluorescence of AGPs recognized by antibody in the vascular bundle sheaths and the secretory ducts in parenchyma.② The Pn and Gs of leaves showed a trend of alternating high and low,and reached the highest or higher level at 90 days after blossom;the Tr reached the highest value at 60 days after blossom and then decreased gradually;and the change trend of Ci was opposite to that of Tr.The contents of glucose and fructose showed a similar trend of gradual increase,and the content of glucose was always higher than that of fructose.The contents of sucrose decreased first and then gradually increased to the highest with the development of leaves,the increase speed and increment of sucrose content were always much higher than those of glucose and fructose,indicating that sucrose was mainly accumulated in leaves.The photosynthesis characteristics and the soluble suger contents of leaf showed obvious variations laws.All the results above indicated that there were some differences in fluorescence intensity of antigen recognized by antibody in different leaf tissues,the mesophyll was the main tissue of AGPs distribution in leaf all along,and AGPs probably participated in protection,drought resistance and nutritional support.关键词
灵武长枣/叶/AGPs/免疫荧光定位/光合特性Key words
Ziziphus jujuba Mill'Lingwuchangzao'/leaf/AGPs/immunofluorescence localization/photosynthesis characteristics分类
农业科技引用本文复制引用
陶珊珊,章英才,王静..灵武长枣叶阿拉伯半乳糖蛋白分布及光合特性[J].农业科学研究,2023,44(4):26-33,8.基金项目
国家级大学生创新创业训练计划项目(G202210749023) （G202210749023）
宁夏自然科学基金项目(2022AAC03087) （2022AAC03087）
