药学研究2023，Vol.42Issue(12)：982-987,993,7.DOI:10.13506/j.cnki.jpr.2023.12.006
基于UPLC-Q-Orbitrap-MS技术的九里香根化学成分分析
Chemical composition analysis of the root of Murraya exotica based on UPLC-Q-Orbitrap-MS
孙璐 1邓仕任 1夏林波 1边星 1杨浩1
作者信息
- 1. 辽宁中医药大学药学院,辽宁 大连 116600
- 折叠
摘要
Abstract
Objective To establish a method for the analysis of the main chemical components in the root of Murraya exotica using UPLC-Q-Orbitrap MS.Methods The analysis was performed on Waters ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm)chromatographic column,with 0.1%formic acid water(A)-0.1%formic acid acetonitrile(B)as the mobile phase for gradient elution.MS1 and MS2 data of samples were collected in positive and negative ion mode,respective-ly.Results By deducing the fragmentation rule of mass spectrometry,comparing with the reference substance,literature data and mass spectrometry database,63 components were preliminarily identified from the root of M.exotica,mainly inclu-ding coumarins,flavonoids,alkaloids,adenosine,etc.Among them,26 components were discovered from M.exotica for the first time.Conclusion This method can provide a basis for elucidating the pharmacological substance basis of root of M.ex-otica.关键词
九里香根/超高效液相串联四极杆静电场轨道阱质谱技术/化学成分/质谱裂解规律Key words
Root of Murraya exotica/UPLC-Q-Orbitrap MS/Chemical composition/Mass fragmentation patterns分类
中医学引用本文复制引用
孙璐,邓仕任,夏林波,边星,杨浩..基于UPLC-Q-Orbitrap-MS技术的九里香根化学成分分析[J].药学研究,2023,42(12):982-987,993,7.
