中国海洋大学学报（自然科学版）2024，Vol.54Issue(2)：33-43,11.DOI:10.16441/j.cnki.hdxb.20230014
3种不同添加物对凡纳滨对虾生长、非特异免疫和抗病力的影响
Effects of Three Additives on Growth Performance,Non-Specific Immunity and Disease Resistance of Litopenaeus vannamei
摘要
Abstract
An experiment was conducted to study the effects of three additives on the growth performance,non-specific immunity and disease resistance of Litopenaeus vannamei.Shrimp were fed with live cells of Clostridium butyricum CBG01(CB group),3%poly-β-hydroxybutyrate(PHB group)and 1%sodium butyrate(BS group)in the basal diets,respectively.The basal diet without additives was used as the control group and the feeding trial lasted for 6 weeks.After feeding trial,the final bodyweight,the non-specific immune parameters in the serum and the relative expression levels of immune-related genes in the hepatopancreas of shrimp were measured.Meanwhile,a Vibrio parahaemolyticus challenge experiment was carried out.Results indicated that the final weight and specific growth rate of shrimp in the CB and PHB groups were significantly improved as compared to the control(P<0.05),especially in the CB group.There was no significant difference between the BS and control groups(P>0.05).The survival rate and feed efficiency of three treatment groups were significantly higher than those of the control group(P<0.05).The activities of lysozyme(LZM),peroxidase(POD),superoxide dismutase(SOD)and total antioxidant capacity(T-AOC)in serum of shrimp of three treatment groups were significantly higher than those of the control group(P<0.05).The activities of alkaline phosphatase(AKP),acid phosphatase(ACP)and phenol oxidase(PO)in serum of the CB group,the activities of AKP,ACP and total nitric oxide synthase(T-NOS)in serum of the PHB group and ACP activity in serum of the BS group were significantly higher than those of the control group(P<0.05).Compared with the control group,the relative expression levels of all immune-related genes(SOD,LZM,proPO,LGBP,HSP70,Imd,Toll,Relish,TOR,4E-BP,eIF4E1α,eIF4E2)in the hepatopancreas of shrimp of the CB group were significantly higher than those of the control group(P<0.05).However,the relative expression levels of Toll gene in the PHB group and Imd,Toll,Relish,eIF4E2 genes in the BS group were not significantly different from those in the control group(P>0.05).The challenge test of V.parahaemolyticus showed that the cumulative mortalities of shrimp in three treatment groups were significantly lower than that in the control group(P<0.05).The results showed that dietary addition of C.butyricum,PHB and sodium butyrate improved the growth performance and non-specific immunity capacity to varying degrees and significantly enhanced the resistance of L.vannamei against V.parahaemolyticus.By comprehensive comparisons,the effects of adding C.butyricum and PHB were basically comparable and to some extent superior to the addition of sodium butyrate.When it is difficult to add live cells of C.butyricum in feed production and processing,it is suggested that appropriately added poly-β-hydroxybutyrate may substitute C.butyricum.关键词
丁酸梭菌/聚β-羟基丁酸酯/丁酸钠/凡纳滨对虾/生长/非特异性免疫/抗病力/副溶血弧菌Key words
Clostridium butyricum/poly-β-hydroxybutyrate/sodium butyrate/Litopenaeus van-namei/growth/non-specific immunity/disease resistance/Vibrio parahaemolyticus分类
农业科技引用本文复制引用
田相利,秦光彩,罗凯,汪仕爽,刘杨,魏聪,王明阳,董双林,刘清兵..3种不同添加物对凡纳滨对虾生长、非特异免疫和抗病力的影响[J].中国海洋大学学报（自然科学版）,2024,54(2):33-43,11.基金项目
国家重点研究发展计划项目(2020YFD0900201,2019YFD0900403) （2020YFD0900201,2019YFD0900403）
青岛市生物制造行业智库联合基金项目(QDSWZK202111)资助Supported by the National Key Research and Development Program of China(2020YFD0900201,2019YFD0900403) （QDSWZK202111）
the Joint Fund of Think Tank for Biomanufacturing Industry of Qingdao(QDSWZK202111) （QDSWZK202111）
