中国海洋大学学报（自然科学版）2024，Vol.54Issue(2)：44-54,11.DOI:10.16441/j.cnki.hdxb.20220467
高温胁迫下刺参H3K9ac特征及HATs、HDACs的表达
The Acetylation Characteristics of H3K9 and the Expression of HATs and HDACs Under Heat Stress in Sea Cucumber(Apostichopus japonicus)
摘要
Abstract
The purpose of this study aims to explore the changes of acetylation levels of H3K9 in Apostiopsis japonicus under heat stress and the effects of HATs and HDACs on the changes.Western blot technique was used to determine the characteristics of H3K9ac at 26℃in A.japonicus.Through the analysis of the genome of A.japonicus,the gene structure and function domain of histone acetylase ajKAT2B,ajKAT5,ajKAT7,ajKAT8 and deacetylase ajSIRT1,ajHDAC1,ajHDAC3,ajHDAC4 were acquired.Phylogenetic trees were constructed by analyzing these 8 genes with those from other species,respectively.The temporal expression levels of the 8 genes in the intestine at 26℃were assessed by qRT-PCR.Western Blot analysis suggested that the abundance of H3K9ac gene transcript increased significantly after 48 h heat stressing,and decreased significantly after 96 h heat stressing(P<0.05),indicating that it may play an important role in regulating gene transcription under high temperature stress.The analysis of gene structure revealed that the evolution of the 8 genes was almost in accordance with the evolution of other species,which showed a high conservativeness in function.The results of qRT-PCR showed that after 48 h of high temperature stress,the mRNA abundances of the four HATs genes increased significantly(P<0.05),and after 96 h heat stress,the expression of ajKAT2B and ajKAT8 decreased to that of control.The expression of ajKAT5 decreased rapidly to the lowest,and the expression of ajKAT7 decreased slightly but was still significantly higher than that of control.Among the four HDACs,The mRNA abundance of ajSIRT1 increased significantly to the highest after 6 h heat stress,and still maintained a higher level than the control group after 48 h heat stress(P<0.05),and then decreased to the control level after 96 h heat stress.AjHDAC1 mRNA abundance decreased significantly after 6 h heat stress(P<0.05),and increased rapidly to the highest after 48 h heat stress(P<0.05),and then decreased to the level of the control group.The mRNA abundance of both ajHDAC3 and ajHDAC4 increased significantly after 6 h heat stress(P<0.05),and then decreased to the level of the control group.The H3K9ac acetylation level of A.japonicus showed obvious dynamic changes under heat stress.HATs and HDACs all showed obvious response at the mRNA level,which may be one of the factors causing the changes of H3K9ac.关键词
刺参/高温胁迫/H3K9ac/组蛋白乙酰转移酶/组蛋白去乙酰化酶Key words
Apostichopus japonicus/heat stress/H3K9ac/histone acetyltransferases/histone deacetylases分类
农业科技引用本文复制引用
张京京,徐冬雪,宋文琦,夏斌,高勤峰..高温胁迫下刺参H3K9ac特征及HATs、HDACs的表达[J].中国海洋大学学报（自然科学版）,2024,54(2):44-54,11.基金项目
国家自然科学基金项目(31902360) （31902360）
山东省自然科学基金项目(ZR2022MC050)资助Supported by the National Natural Science Foundation of China(31902360) （ZR2022MC050）
the Natural Science Foundation of Shandong Province(ZR2022MC050) （ZR2022MC050）
