中国海洋大学学报（自然科学版）2024，Vol.54Issue(2)：55-62,8.DOI:10.16441/j.cnki.hdxb.20220269
副溶血弧菌现场检测系统的研发及应用
Development and Application of Point-of-Care Test System for Detection of Vibrio parahaemolyticus
摘要
Abstract
In order to meet the requirements of the on-site detection of Vibrio parahaemolyticus in seafood,an point-of-care test system for V.parahaemolyticus based on electrochemical detection and loop-mediated isothermal amplification was established in this study.The core circuit of the device is only 7 cm×4 cm in size and weighs 30 g.The system is easy to carry.The detection result can be obtained within 30 min.The detection limit of V.parahaemolyticus in samples can reach 103 CFU/mL.At the same time,the system is used to detect V.parahaemolyticus in the stained shrimp samples,with a detection limit as low as 3.6×103 CFU/g.With the progress of the nucleic acid amplification reaction,the pH of the nucleic acid amplification system decreased.The pH sensitive electrode prepared in this study can monitor the pH change of the nucleic acid amplification system in real time with an accuracy of 0.016 pH units.Therefore,the detection limit of this system can be consistent with that of fluorescence detection.The system has the advantages of portability,rapidness,high sensitivity,low cost,on-site detection among others,which is conducive to sink downward to the basic level of molecular diagnosis technology in food safety,and meets the important national strategic needs of public health safety.关键词
副溶血弧菌/即时检测/环介导等温扩增/电化学检测/便携设备Key words
Vibrio parahaemolyticus/immediate detection/loop mediated isothermal amplification/electrochemical detection/portable equipment分类
生物科学引用本文复制引用
李琪,高倩,田浩,石超..副溶血弧菌现场检测系统的研发及应用[J].中国海洋大学学报（自然科学版）,2024,54(2):55-62,8.基金项目
国家自然科学基金项目(2018YFE0113300) （2018YFE0113300）
山东省自然科学基金项目(ZR2020KH030)资助Supported by the National Natural Science Foundation of China(2018YFE0113300) （ZR2020KH030）
the Natural Science Foundation of Shandong Province(ZR2020KH030) （ZR2020KH030）
