中国海洋大学学报（自然科学版）2024，Vol.54Issue(2)：84-88,105,6.DOI:10.16441/j.cnki.hdxb.20220128
海洋细菌Pseudoalteromonas sp.01中新型褐藻胶裂解酶AlyPC1的研究
Study on the New Alginate Lyase AlyPC1 from Marine Bacterium Pseudoalteromonas sp.01
摘要
Abstract
To explore new alginate lyases and develop them as the tool enzymes for the preparation of alginate oligosaccharides,an alginate lyase AlyPC1 from Pseudoalteromonas sp.01 was characterized.On the basis of bioinformatics analysis,the clone,recombinant expression,and enzymatic properties of AlyPC1 were studied.Sequence alignment showed that AlyPC1 belonged to the fifth subfamily of PL7.The optimal temperature of AlyPC1 was 35℃,and over 80%of the initial activity was retained after in-cubation at 5～30℃for 1 h.The optimal pH value of AlyPC1 was 8.0 in Tris-HCl buffer,and the en-zyme activity of AlyPC1 was retained over 90%in the range of pH 7.5 to 9.0.It was proved that AlyPC1 showed good temperature stability and broad pH tolerance.AlyPC1 exhibited the maximum ac-tivity in the buffer with 200 mmol/L NaCl.EDTA and metal ions,such as Mn2+,Zn2+,and Ni2+,sig-nificantly inhibited the enzyme activity of AlyPC1.AlyPC1 was a bifunctional alginate lyase,which could cleave alginate,polyG,and polyM.Meanwhile,AlyPC1 degraded alginate into alginate oligosac-charides with the composition of disaccharides,trisaccharides,and tetrasaccharides.关键词
褐藻胶裂解酶/褐藻寡糖/内切酶/双功能/稳定性Key words
alginate lyase/alginate oligosaccharides/endolysis/bifunctional/stability分类
生物科学引用本文复制引用
张红秀,刘晓华,刘伟治..海洋细菌Pseudoalteromonas sp.01中新型褐藻胶裂解酶AlyPC1的研究[J].中国海洋大学学报（自然科学版）,2024,54(2):84-88,105,6.基金项目
国家重点研究发展计划项目(2018YFC0311105)资助Supported by the National Key Research and Development Program of China(2018YFC0311105) （2018YFC0311105）
