青岛大学学报（医学版）2023，Vol.59Issue(6)：791-795,5.DOI:10.11712/jms.2096-5532.2023.59.189
芝麻素对氧糖剥夺损伤大鼠皮质神经元的作用及机制
EFFECT OF SESAMIN ON CORTICAL NEURON INJURY INDUCED BY OXYGEN-GLUCOSE DEPRIVATION IN RATS AND RE-LATED MECHANISM
摘要
Abstract
Objective To investigate whether sesamin(SSM)exerts a neuroprotective effect on rat cortical neurons with oxygen-glucose deprivation(OGD)injury through the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/nuclear factor-erythroid 2-related factor 2(Nrf2)signaling pathway.Methods Primary cortical neurons of Sprague-Dawley rats were cultured for 8 days to construct an in vitro model of cerebral ischemia.CCK-8 assay was used to observe the effect of different concentrations of SSM on the viability of neurons,and Western blotting was used to measure the protein expression levels of phosphorylated Akt(p-Akt),Akt,Nrf2,and heme oxygenase-1(HO-1)after treatment with the optimal concentration of SSM.Results Com-pared with the Control group,the OGD group had a significant reduction in the viability of neurons,and SSM treatment at different concentrations could increase the viability of neurons after OGD injury(F=35.93,P<0.01),with the most obvious effect at the concentration of 25μmol/L.There were significant differences between groups in the expression levels of the oxidative stress-rela-ted proteins p-Akt,Nrf2,and HO-1(F=23.47-32.21,P<0.01).Compared with the OGD group,the OGD+SSM group had significantly upregulated protein expression of p-Akt,Nrf2,and HO-1(P<0.05),andcomparedwiththe OGD+SSMgroup,the OGD+SSM+LY294002 group had inhibited upregulation of p-Akt,Nrf2,and HO-1 proteins(P<0.05).There was no significant difference in Akt between groups(P>0.05).Conclusion SSM plays a neuroprotective role by activating the PI3K/Akt/Nrf2 signaling pathway and increasing the viability of neurons after OGD injury.关键词
芝麻脂素/大脑皮质/神经元/脑缺血/神经保护Key words
sesamin/cerebral cortex/neurons/brain ischemia/neuroprotection分类
基础医学引用本文复制引用
陈淑君,何佳林,万芪..芝麻素对氧糖剥夺损伤大鼠皮质神经元的作用及机制[J].青岛大学学报（医学版）,2023,59(6):791-795,5.基金项目
国家自然科学基金资助项目(82071385) （82071385）
