青岛大学学报（医学版）2023，Vol.59Issue(6)：796-801,6.DOI:10.11712/jms.2096-5532.2023.59.196
骨髓间充质干细胞旁分泌KGF对LPS诱导小鼠肺上皮细胞损伤的修复作用
REPAIR EFFECT OF KERATINOCYTE GROWTH FACTOR SECRETED BY BONE MARROW MESENCHYMAL STEM CELLS THROUGH A PARACRINE MECHANISM ON LIPOPOLYSACCHARIDE-INDUCED MOUSE LUNG EPITHELIAL CELL DAMAGE
摘要
Abstract
Objective To investigate the repair effect of keratinocyte growth factor(KGF)secreted by bone marrow mesenchymal stem cells(BMSC)through a paracrine mechanism on lipopolysaccharide(LPS)-induced alveolar epithelial damage in acute respiratory distress syndrome(ARDS),as well as the role of receptor for advanced glycation end products(RAGE)from alveolar epithelial cells on the repair of LPS-induced alveolar epithelial damage by KGF secreted by BMSC.Methods Mouse lung epithelial MLE12 cells were used to establish a model of LPS-induced damage,and an indirect co-culture model was established for BMSC and MLE12 in vitro.The subjects were divided into control group,LPS group,MSC-control group,MSC-LPS group,KGF group,and anti-RAGE group.After 1 and 7 days of culture,Western blot was used to measure the content of RAGE and zonula oc-cluden-1(ZO-1)in MLE12 cells,and ELISA was used to measure the content of KGF and RAGE in the conditioned medium of cells.Results BMSC could increase the protein expression of ZO-1 in lung epithelium damaged by LPS(F=172.40,146.80,P<0.01;t=14.91-67.40,P<0.05).During the repair of lung epithelium damaged by LPS with BMSC,there were increases in the content of KGF in conditioned medium(F=45.51,2 359.00,P<0.01;t=14.87-73.25,P<0.05)and the expression of RAGE in MLE12 cells(F=49.90-275.60,P<0.01;t=11.02-57.84,P<0.05).Conclusion BMSC can repair the damage of MLE12 cells induced by LPS,and KGF secreted by BMSC through a paracrine mechanism plays an important role in such repair and may help to achieve the repair effect by regulating the expression of RAGE.关键词
间质干细胞/呼吸窘迫综合征/成纤维细胞生长因子10/高级糖化终产物受体/脂多糖类Key words
mesenchymal stem cells/respiratory distress syndrome/fibroblast growth factor 10/receptor for advanced glycation end products/lipopolysaccharides分类
医药卫生引用本文复制引用
王以诺,蔡施霞,李连弟,吕坤聚..骨髓间充质干细胞旁分泌KGF对LPS诱导小鼠肺上皮细胞损伤的修复作用[J].青岛大学学报（医学版）,2023,59(6):796-801,6.基金项目
国家自然科学基金青年科学基金项目(81701879) （81701879）
