青岛大学学报（医学版）2023，Vol.59Issue(6)：808-813,6.DOI:10.11712/jms.2096-5532.2023.59.174
花生四烯酸对大鼠缺血性脑损伤的作用及机制
EFFECT OF ARACHIDONIC ACID ON ISCHEMIA-REPERFUSION INJURY IN RATS AND ITS MECHANISM
摘要
Abstract
Objective To investigate whether arachidonic acid(AA)exerts a protective effect against ischemic brain inju-ry in rats via the human silencing regulatory protein 6(SIRT6)-human phosphatase and tensin homolog deleted on chromosome 10(PTEN)pathway.Methods We constructed an in vivo model of middle cerebral artery embolism(MCAO)in rats and an ex vi-vo model of cortical neuronal oxygen glucose deprivation(OGD).The expression levels of PTEN and SIRT6 proteins in cortical neurons were measured using Western blot and immunofluorescence staining.Brain infarct volume was observed in rats using 2,3,5-triphenyltetrazolium chloride staining.The survival of cortical neurons was examined using the Cell Counting Kit-8.Results AA supplementation after MCAO reduced cerebral infarct volume(F=367.80,P<0.05).AA supplementation after OGD injury alleviated cortical neurons injury(F=175.40,P<0.05)by upregulating SIRT6 expression and downregulating PTEN expression(F=11.67,14.85,P<0.05).Administration of OSS_128167(SIRT6 inhibitor)blocked AA-induced downregulation of PTEN(F=15.63,P<0.05)and increased neuronal survival(F=175.20,P<0.05).Conclusion AA exerts neuroprotective effects through regulating the SIRT6-PTEN signaling pathway to ameliorate ischemic brain injury in rats.关键词
花生四烯酸/缺氧缺血,脑/PTEN磷酸水解酶/抗衰老酶/神经保护Key words
arachidonic acid/hypoxia-ischemia,brain/PTEN phosphohydrolase/sirtuins/neuroprotection分类
医药卫生引用本文复制引用
李晓华,万芪..花生四烯酸对大鼠缺血性脑损伤的作用及机制[J].青岛大学学报（医学版）,2023,59(6):808-813,6.基金项目
国家自然科学基金资助项目(82071385) （82071385）
