青岛大学学报（医学版）2023，Vol.59Issue(6)：814-820,7.DOI:10.11712/jms.2096-5532.2023.59.179
染色质调控因子相关基因在乳癌预后评估中的价值
VALUE OF CHROMATIN REGULATOR-RELATED GENES IN EVALUATING THE PROGNOSIS OF BREAST CANCER
摘要
Abstract
Objective To construct a prognostic model of chromatin regulator(CR)-related genes,and to investigate the value of CR-related genes in evaluating the prognosis of breast cancer.Methods A difference analysis was performed in the Gene Expression Omnibus database to screen for the differentially expressed CR-related genes.Univariate Cox regression analysis and LASSO regression analysis were used to establish and validate the prognostic model based on CR-related genes.A total of 30 pairs of breast cancer tissue samples and adjacent tissue samples were selected,and RT-qPCR was used to verify the expression of key CR-related genes.Results According to the results of the difference analysis,a prognostic model of breast cancer patients was established based on four CR-related genes(MORF4L1,NCOA4,TTK,and JMJD4),and the patients were divided into high-and low-risk groups according to the cut-off value of the risk model.Survival analysis showed that the high-risk group tended to have a poor prognosis.The results of RT-qPCR showed that there were reductions in the expression of MORF4L1 and NCOA4 and increases in the expression of TTK and JMJD4 in breast cancer tissue,which was consistent with the results of the difference analysis.Conclusion The prognostic model based on the above four CR-related genes can effectively predict the prognosis of breast cancer patients.关键词
乳腺肿瘤/染色质调控因子/列线图/预后Key words
breast neoplasms/chromatin regulators/nomograms/prognosis分类
医药卫生引用本文复制引用
张蔚然,吕志栋,宋洪明,夏菁,郇喻淇,孔滨..染色质调控因子相关基因在乳癌预后评估中的价值[J].青岛大学学报（医学版）,2023,59(6):814-820,7.基金项目
中国博士后科学基金资助项目(2020M672001) （2020M672001）
吴阶平医学基金会临床科研专项资助基金(320.6750.2022-03-39) （320.6750.2022-03-39）
