通腑理肺汤对脓毒症肠屏障损伤大鼠肠黏膜组织闭锁小带蛋白-1、闭合蛋白mRNA及蛋白表达的影响OACSTPCD

Objective:To explore the effect of Tongfu Lifei decoction(TFL)on the mRNA genes and protein expression of ZO-1 and Occludin in intestinal mucosa tissue of sepsis rats with intestinal barrier injury.Methods:Randomly selected 40 purebred SD rats and divided them into four groups:Sham surgery group,model group,low dose TFL group,and high dose TFL group,with 10 rats in each group.Using the method of cecal ligation and perfo-ration(CLP)to create a rat model of sepsis intestinal barrier injury.In the sham operation group,only the cecum was exposed without perforation.TFL low-dose group was given 3.6 g/kg body weight TFL by gavage after successful modeling.After modeling using the same method,the high-dose TFL group was treated with 7.2 g/kg body weight TFL by gavage.The model group and sham operation group were given 10 ml/kg of distilled water by gavage,and each group was intervened once a day for a total of 7 days.After 7 days,the rats in each group were killed,and the in-testinal mucosa tissues were isolated.ZO-1 and Occludin mRNA genes were detected by RT-qPCR,and the expres-sion of ZO-1 and Occludin protein was detected by Western blot.At the same time,took out the intestinal mucosa at the same part of each group,and observed the pathological and ultrastructural changes of intestinal mucosa under light and transmission electron microscope.Results:The mRNA genesand protein expressions of ZO-1 and Occludin in intestinal mucosal tissue of model group rats were significantly decreased compared with those of sham operation group(P<0.05).The mRNA genes and protein expressions of ZO-1 and Occludin in the intestinal mucosa of rats in the low-dose and high-dose TFL group were significantly increased compared to the model group(P<0.05).The histopathological observation of intestinal mucosa under light microscope showed that the edema of intestinal mucosal epithelial cells,the damage of intestinal mucosal villi,the edema of basal layer and lamina propria,the infiltration of lymphocytes and neutrophils were significantly reduced in the low and high dose groups of TFL.The ultrastructure of intestinal mucosa under transmission electron microscope also showed that the microvilli in the low and high dose groups of TFL were dense and regular,the intestinal cells were serrated and interlocked,the mitochondria were clear,and the tight junction was slightly damaged.Conclusion:The reduction of pathological damage in a sepsis induced in-testinal barrier injury rat model may be due to TFL upregulating the expression of tight junction ZO-1 and Occludin mRNA genes and proteins in intestinal mucosal tissue,improving the tight junction effect between intestinal epithelial cells.