青岛大学学报（医学版）2023，Vol.59Issue(6)：860-866,7.DOI:10.11712/jms.2096-5532.2023.59.203
LINC00657对结直肠癌细胞恶性行为影响及机制
EFFECTS OF LINC00657 ON MALIGNANT BEHAVIOR OF COLORECTAL CANCER CELLS AND UNDERLYING MECHANISMS
摘要
Abstract
Objective To investigate the effects of long non-coding RNA(LINC00657)on the proliferation,invasion,and migration of colorectal cancer(CRC)cells by regulating miR-26b and its target gene mucosa-associated lymphoid tissue lym-phoma translocation gene 1(MALT1).Methods Reverse transcription polymerase chain reaction(RT-PCR)was used to mea-sure the expression of LINC00657 in normal intestinal epithelial cells(NCM460)and CRC cell lines(HT29,HCT116,and SW620).HT29 cells were transfected with shRNA-LIC00657 or shRNA-CON to analyze the effects of silencing LINC00657 on the proliferation,invasion,and migration of cancer cells.The targeted relationship between LINC00657,miR-26b,and MALT1 was verified by dual-luciferase reporter assay.HT29 cells were separately transfected with shRNA-LINC00657 alone,shRNA-LINC00657+miR-26b inhibitor,and shRNA-LINC00657+pcDNA-MALT1 to analyze the effects of LINC00657 on cell prolifera-tion,invasion,and migration through the miR-26b/MALT1 axis.Cell proliferation was determined using Cell Counting Kit-8.Cell invasion and migration was determined using Transwell assay.The expression level of MALT1 mRNA was measured by RT-PCR.The expression level of MALT1 protein was measured by Western blot.Results The expression levels of LINC00657 in CRC cell lines(HT29,HCT116,and SW620)were significantly higher than that in normal intestinal epithelial cells(NCM460)(F= 30.267,P<0.05).Silencing LINC00657 significantly inhibited the proliferation,invasion,and migration of HT29 cells(t=9.123-18.456,P<0.05).The dual-luciferase reporter assay showed that LINC00657 targeted miR-26b to downregulate its expression,and miR-26b negatively regulated the expression of MALT1.Silencing LINC00657 expression significantly inhibited the expression of MALT1 mRNA and protein in HT29 cells(F = 15.893,17.231,P<0.05).Compared with those transfected with shRNA-LINC00657 alone,the cells transfected with shRNA-LINC00657+miR-26b inhibitor or shRNA-LINC00657+MALT1 overexpression vector had significantly up-regulated expression of MALT1 mRNA and protein(F=15.893,17.231,P<0.05)and significantly increased abilities of cell proliferation,invasion,and migration(F=4.783-8.893,P<0.05).Conclusion LINC00657 is highly expressed in CRC cells,which can promote the proliferation,invasion,and migration of cancer cells by regulating the miR-26b/MALT1 axis.关键词
结直肠肿瘤/RNA,长链非编码/黏膜相关淋巴样组织淋巴瘤易位1蛋白/细胞增殖/肿瘤浸润Key words
colorectal neoplasms/RNA,long noncoding/mucosa-associated lymphoid tissue lymphoma translocation 1 protein/cell proliferation/neoplasm invasiveness分类
医药卫生引用本文复制引用
张生军,赵阿静,张安瑞,李雅微,李小宝,刘敏丽..LINC00657对结直肠癌细胞恶性行为影响及机制[J].青岛大学学报（医学版）,2023,59(6):860-866,7.基金项目
陕西省科技厅重点研发计划项目(2021SF-310) （2021SF-310）
