陕西中医2024，Vol.45Issue(1)：17-22,6.DOI:10.3969/j.issn.1000-7369.2024.01.004
养阴凉血方对过敏性紫癜患儿血清诱导内皮细胞损伤的保护作用
Protective effect of Yangyin Liangxue recipe on endothelial cells induced by serum in children with Henoch-schnlein purpura
摘要
Abstract
Objective:To investigate the protective effect and mechanism of Yangyin Liangxue recipe on endo-thelial cells induced by serum of children with Henoch-schonlein purpura(HSP).Methods:HUVECs were used as the research object,the serum of healthy children and children with HSP were to intervene,and different concentra-tions of Yangyin Liangxue recipe were added.They were divided into blank control group,normal control group,HSP group and Yangyin Liangxue recipe(0.5,1 g/ml)group.The supernatant was collected after 24 hours of culture.Cell morphology was observed under inverted microscope.The levels of TNF-α,IL-8,IgA,CD89 in the supernatant of each group were determined by ELISA.Western blot was used to detect the expression of PI3 K protein in each group.Results:The levels of TNF-α,IL-8,IgA,CD89 and the expression PI3 K protein in the supernatant of each group were significantly higher in HSP group than in the blank and normal control group(all P<0.05),and the number of cells was decreased and the morphology was shrunk.Compared with the HSP group,the levels of TNF-α,IL-8,IgA,CD89 and PI3K protein expression in the Yangyin Liangxue recipe two groups were significantly decreased(all P<0.05),and the trend of cell number reduction and morphological shrinkage was improved.Conclusion:Yangyin Liangxue recipe can treat Henoch-schonlein purpura and its mechanism may be related to regulating the secretion of cytokines such as TNF-α,IL-8,IgA and CD89 and reducing the phosphorylation level of PI3K protein in PI3K/AKT/NF-κB signaling pathway.关键词
过敏性紫癜/人脐静脉内皮细胞/养阴凉血方/炎症因子/免疫球蛋白/PI3K蛋白Key words
Henoch-schonlein purpura/Human umbilical vein endothelial cells/Yangyin Liangxue recipe/In-flammatory factor/Immunoglobulin A/PI3K protein分类
医药卫生引用本文复制引用
何分,史艳平,李霞,赵珍珍,邱锐,赵爱霞..养阴凉血方对过敏性紫癜患儿血清诱导内皮细胞损伤的保护作用[J].陕西中医,2024,45(1):17-22,6.基金项目
陕西省自然科学基础研究计划项目(2022JM-534) （2022JM-534）
陕西省中医药管理局科研项目(ZYJXG-L23007) （ZYJXG-L23007）
西安市卫生健康委员会科研项目(2023QN14) （2023QN14）
