陕西中医2024，Vol.45Issue(1)：23-28,6.DOI:10.3969/j.issn.1000-7369.2024.01.005
基于MAPK/ERK通路探讨益肾调经丸对大鼠多囊卵巢综合征的作用和机制研究
Effect and mechanism of Yishen Tiaojing pill on polycystic ovarian syndrome rats based on MAPK/ERK pathway
摘要
Abstract
Objective:To explore the effect of Yishen Tiaojing pill on polycystic ovary syndrome(PCOS)model rats.Methods:70 female SD rats were randomly divided into normal group,model group,normal saline group,Diane 35 group,Yishen Tiaojing pill low,medium,and high groups,with 10 rats in each group.Using letrozole and high-fat feed for modeling,after successful modeling,each treatment group and normal saline group were treated with corre-sponding doses of medication for 28 days.ELISA method was used to detect serum T,FSH,LH levels,and LH/FSH was calculated.Observed changes in ovarian morphology.HE staining was used to observe the ovaries.Real time PCR detection of CYP17 A1 and CYP19 A1.Western blot was used to detect the protein expression of p-ERK1/2.Results:Compared with the normal group,polycystic changes can be observed in the ovaries of model group rats,ser-um T and LH levels increased,FSH decreased,LH/FSH increased,and the ovaries showed polycystic changes.The expression of CYP17 A1 mRNA increased,CYP19 A1 mRNA decreased,and p-ERK1/2 protein expression increased in ovarian(P<0.05).Compared with the model group,there was no significant change in all detection indicators in the normal saline group.Alleviation of polycystic ovary changes in rats of each treatment group,serum T and LH lev-els were reduced,FSH was increased(P<0.05),polycystic ovary changes were alleviated,CYP17 A1 mRNA expres-sion was reduced in ovarian,and CYP19 A1 mRNA expression was increased(P<0.05).The p-ERK1/2 protein ex-pression level in the treatment group was improved to varying degrees,The improvement of each treatment group with Yishen Tiaojing pill was statistically significant(P<0.05),while the change in the Diane 35 group was not sta-tistically significant(P>0.05).Conclusion:Yishen Tiaojing pill can improve sexual hormone levels and alleviate polycystic ovary changes in PCOS rats.Yishen Tiaojing pill may reduce CYP17 A1,upregulate CYP19 A1 expression,and lower androgen levels through the MAPK/ERK signaling pathway,thereby improving polycystic ovary changes and hyperandrogenicity in PCOS rats.关键词
多囊卵巢综合征/MAPK/ERK通路/益肾调经丸/补肾活血法/高雄激素血症/来曲唑/大鼠Key words
Polycystic ovary syndrome/MAPK/ERK pathway/Yishen Tiaojing pill/Tonifying kidney and ac-tivating blood method/Hyperandrogenism/Letrozole/Rat分类
医药卫生引用本文复制引用
刘雯星,陈怡,黄缨,殷秀琴,周芸..基于MAPK/ERK通路探讨益肾调经丸对大鼠多囊卵巢综合征的作用和机制研究[J].陕西中医,2024,45(1):23-28,6.基金项目
湖北省自然科学基金资助项目(2022CFB351) （2022CFB351）
湖北省医学领军人才培养工程专项经费资助项目[(2019)47] （2019）
湖北省荆州市中医药研究所自主选题资助项目(ZZXT2022Y11) （ZZXT2022Y11）
