青岛大学学报（医学版）2023，Vol.59Issue(6)：894-897,4.DOI:10.11712/jms.2096-5532.2023.59.202
丹参葛根提取物对阿霉素引发小鼠心力衰竭的作用
EFFECT OF SALVIA MILTIORRHIZA AND RADIX PUERARIAE EXTRACT ON DOXORUBICIN-INDUCED HEART FAILURE IN MICE
摘要
Abstract
Objective To explore the cardioprotective effect of Salvia miltiorrhiza-Radix puerariae extract(DG)on mice with heart failure induced by doxorubicin(DOX-HF mice).Methods In the experiment,32 C57BL/6J mice(6 weeks old,male)were randomly divided into four groups:control group(group A),model group(group B),low-dose DG group(group C),and high-dose DG group(group D)(n=8 per group).Mice form groups B,C,and D received an intraperitoneal injection of DOX for 4 weeks(5 mg/kg per week).Meanwhile,mice in groups C and D were intragastrically given DG water solution once daily at the beginning of modeling with doses of 50 and 100 mg/kg,respectively.Mice in groups A and B were intragastrically given the same dose of normal saline daily.The changes in cardiac mechanical function were evaluated by echocardiography;myocardial his-topathological changes were observed by hematoxylin-eosin(HE)staining and Masson staining;enzyme-linked immunosorbent as-say(ELISA)was used to measure levels of serum N-terminal pro-brain natriuretic peptide(NT-proBNP),cardiac troponin I(cTnI),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6).Results Echocardiography data showed that compared with group A,group B had significantly decreased left ventricular ejection fraction and left ventricular fractional shortening and sig-nificantly increased left ventricular end-diastolic volume and left ventricular end-systolic volume.Above phenomena that occurred in group B were significantly reversed in groups C and D(F=7.66-29.30,P<0.05).Furthermore,the data from HE staining showed that significantly abnormal myocardial tissue structure characterized by disordered and edematous myocardial cells especial-ly with se-rious vacuolation was observed in group B compared with group A.Meanwhile,above morphological changes were alle-viated with DG treatment in mice from groups C and D.Besides,the data from Masson staining showed that collagen deposition was significantly increased in group B compared with group A but was significantly reduced in groups C and D compared with group B.Additionally,ELISA data showed that compared with group A,group B had significantly increased levels of serum NT-proBNP,cTnI,TNF-α,and IL-6,while these cytokines were significantly de-creased in mice from groups C and D compared with those from group B(F=17.54-32.59,P<0.05).Conclusion DG presents a cardioprotective effect on DOX-HF mice.关键词
多柔比星/心力衰竭/丹参葛根提取物/炎症Key words
doxorubicin/heart failure/Salvia miltiorrhiza-Radix pueraria extract/inflammation分类
医药卫生引用本文复制引用
张梦瑶,张咪,韩彦弢,赵文文..丹参葛根提取物对阿霉素引发小鼠心力衰竭的作用[J].青岛大学学报（医学版）,2023,59(6):894-897,4.基金项目
山东省中医药科技项目(2021M137) （2021M137）
