肝郁气滞、肝胆湿热型胆囊胆固醇沉着症动物模型建立与评价OACSTPCD

Objective:Establish and evaluate a mouse model of gallbladder cholesterol deposition with liver stag-nation and qi stagnation,liver and gallbladder dampness heat syndrome,in order to provide model support for in-depth research on the pathogenesis,pathological changes,and exploration of clinical treatment plans of this disease.Methods:Overall,48 C57 BL/6J male mice were randomly divided into a blank control group(WT),a 3-week model-ing group(LD-3),a 6-week modeling group(LD-6),and a 9-week modeling group(LD-9),with 12 mice in each group.The WT group was fed with regular feed and normal drinking water,while the modeling group was fed with high fat and cholesterol feed and normal drinking water for 3,6,and 9 weeks,respectively.During the modeling peri-od,the general situation of mice was observed daily.After 3,6,and 9 weeks of anesthesia,the animals were eutha-nized,and blood was collected from the orbit to detect the total cholesterol(TC)and triglyceride(TG)levels in ser-um.Tissue samples were taken to observe the lipid deposition in the gallbladder using Nile red staining,and bile com-ponents were analyzed using infrared spectroscopy.Cholesterol metabolism indicators:Immunohistochemical method was used to detect the protein expression of ATP binding cassette transporter A group 1(ABCA1)in the liver,and the model was evaluated.Results:Compared with the WT group,the weight of the model mice in each group increased significantly(P<0.05).The serum TC level significantly increased(P<0.05),while the serum TG level signifi-cantly decreased(P<0.05).Significant lipid deposition in the gallbladder and increased bile turbidity.The expression of ABCA1 protein in the liver was significantly increased.Conclusion:This study successfully established a mouse gallbladder cholesterol deposition model construction method using high fat and high cholesterol feed and normal drinking water for 9 weeks,which is in line with clinical characteristics.And successfully established the disease syn-drome types of liver stagnation and qi stagnation,liver and gallbladder dampness heat syndrome,which can provide a stable animal model for subsequent research on the pathogenesis and treatment methods of gallbladder cholesterol deposition.