蓝刺头对去卵巢大鼠血清P1NP、β-CTX及TNF-α、IL-1β、IL-6的影响OACSTPCD

Objective:To study the effects of Eechinops latifolius on bone metabolic markers P1 NP and β-CTX in ovariectomized rats.Methods:Seventy-two 4-month-old SD SPF grade female rats were randomly divided into sham group,ovariectomized osteoporosis model group,Mongolian medicine Cyanostis high-dose group,medium-dose group,low-dose group and Epimedium group,12 rats in each group.Except sham operation group,the other groups were treated with ovary removal and castration to establish postmenopausal osteoporosis model.Rats in sham opera-tion group and model group were fed normal saline for 90 days after operation,and rats in each treatment group were intragastric with corresponding drugs once a day for 90 days.24 h after the last administration,the rats were killed,and the femoral bone mineral density of the rats was determined by Micro-CT,and the serum levels of P1 NP,β-CTX,TNF-α,IL-1βand IL-6 were detected by enzyme-linked immunoassay.Results:Compared with sham group,the proxi-mal femur BMD of OVX group was significantly decreased,and the serum contents of P1 NP,β-CTX,TNF-α,IL-1β and IL-6 were significantly increased(P<0.05).Compared with OVX group,BMD was increased and serum con-tents of P1 NP,β-CTX,TNF-α,IL-1βand IL-6 were decreased in treatment group(P<0.05).Conclusion:Mongolian medicine Lanacitou can decrease the contents of P1 NP andβ-CTX in ovariectomized rats,and at the same time de-crease the concentrations of TNF-α,IL-1βand IL-6,thereby increasing bone content and decreasing bone absorption.