饲料研究2023，Vol.46Issue(22)：49-54,6.DOI:10.13557/j.cnki.issn1002-2813.2023.22.010
复方中草药对长江鲟幼鱼免疫、抗氧化及抗病力的影响
Effect of compound Chinese herbal medicine on immunity,antioxidant and disease resistance of juvenile Acipenser dabryanus
摘要
Abstract
The experiment was to study the effect of compound Chinese herbal medicine on the immunity,antioxidant and disease resistance of juvenile Acipenser dabryanus.A total of 360 juvenile Acipenser dabryanus with a body weight of(537.68±25.47)g and a body length of(46.27±5.71)cm were randomly divided into four groups,with three replicates in each group and 30 fish in each replicate.The fish in the control group were fed with a basic feed,and the experimental groups were fed with a basic feed supplemented with 0.5%,1.0%and 1.5%compound Chinese herbal medicine,respectively.The experiment lasted for 21 d.On 14 and 21 d of the experiment,20 juvenile Acipenser dabryanus were randomly selected from each group,and Aeromonas hydrophila was used for challenge test.The results showed that compared with the control group,the serum IgM content in the 1.5%experimental group was significantly increased on 7 d(P<0.05).On 14 d,serum IgG and IgM content in the 1.0%experimental group and the 1.5%experimental group were significantly increased(P<0.05).On 21 d,the content of IgG and IgM in the 1.5%experimental group were higher than those in the control group.Compared with the control group,the content of serum complement C4 and AMP and the activitiy of LSZ and AKP in the 1.5%experimental group were significantly increased on 7,14 d(P<0.05).On 21 d,the serum AMP content of the 1.5%experimental group was still significantly higher than those in the control group(P<0.05).Compared with the control group,the serum SOD activity of the 1.5%experimental group was significantly increased on 7,14,21 d(P<0.05).On 14 d,the activitiy of CAT and POD in the serum of the 1.5%experimental group was significantly increased(P<0.05),while the content of MDA was significantly decreased(P<0.05).On 14,21 d,the serum GSH-Px activity of the 1.5%experimental group was significantly increased(P<0.05).On 7,14 d,serum T-AOC in the 1.5%experimental group was significantly increased(P<0.05).The mortality of the 1.0%experimental group and the 1.5%experimental group on 14,21 d was significantly lower than that of the control group.The study indicates that the compound Chinese herbal medicine can improve the immunity,antioxidant and disease resistance of juvenile Acipenser dabryanus,and a course of treatment with a 1.5%supplement for 14～21 d is more effective.关键词
长江鲟/中草药/免疫/抗氧化/抗病力Key words
Acipenser dabryanus/Chinese herbal medicine/immunity/antioxidant/disease resistance分类
农业科技引用本文复制引用
张建明,张德志,田甜,舒德斌..复方中草药对长江鲟幼鱼免疫、抗氧化及抗病力的影响[J].饲料研究,2023,46(22):49-54,6.基金项目
中国长江三峡集团有限公司科技计划项目(项目编号:WWKY-2021-0003、JG/18001B) （项目编号:WWKY-2021-0003、JG/18001B）
