饲料研究2023，Vol.46Issue(22)：119-126,8.DOI:10.13557/j.cnki.issn1002-2813.2023.22.022
QuEChERS-超高效液相色谱-串联三重四级杆质谱法同时测定玉米秸秆饲料中20种真菌毒素
Simultaneous determination of 20 kinds of mycotoxins in maize straw feed by QuEChERS-ultra performance liquid chromatography-triple quadrupole mass spectrometry
摘要
Abstract
The experiment was to establish an analytical method for the simultaneous determination of 20 kinds of mycotoxins in maize straw feed by QuEChERS-UPLC-MS/MS.The samples were extracted with acetonitrile-acetic acid(95∶5),and the supernatant was purified by anhydrous MgSO4,PSA and C18.Then an appropriate amount of supernatant was blown to dry with nitrogen.The samples were reconstituted with methanol-water-acetic acid(30∶70∶0.1),passed through a PTFE filter membrane,and separated on a C18 chromatographic column.Using the multiple reaction monitoring(MRM)scan mode to monitor the target compounds and the results were quantified by the external standard method.The results showed that the linear ranges of the 20 mycotoxins were good,and the correlation coefficients(R2)were greater than 0.99.The limits of detection were 0.15～3.10 μg/kg,with limits of quantification were 0.49～10.33 μg/kg.The recoveries at the three levels were within the range of 70.4%～118.3%,with relative standard deviations of 1%～10%(n=6).The study indicates that the method was easy to operate,reproducible and highly accurated and sensitive,which can be used for routine screening of mycotoxins in corn stalk feed.关键词
QuEChERS/超高效液相色谱-串联三重四级杆质谱法/玉米秸秆饲料/真菌毒素Key words
QuEChERS/UPLC-MS/MS/maize straw feed/mycotoxins分类
农业科技引用本文复制引用
柳婷婷,张巍巍,史晓梅,翟晨,胡利明,钱承敬,张晓琳..QuEChERS-超高效液相色谱-串联三重四级杆质谱法同时测定玉米秸秆饲料中20种真菌毒素[J].饲料研究,2023,46(22):119-126,8.基金项目
国家重点研发计划"粮油原料化学污染物数据库及风险预测模型的建立与应用示范(项目编号:2019YFC1605301)" （项目编号:2019YFC1605301）
