世界中医药2023，Vol.18Issue(21)：3060-3064,5.DOI:10.3969/j.issn.1673-7202.2023.21.010
祛风宣肺方通过背根神经反射降低咳嗽敏感性的作用机制研究
Qufeng Xuanfei Decoction Reduces Cough Sensitivity via Dorsal Root Reflex
摘要
Abstract
Objective:To investigate the effects of Qufeng Xuantei Decoction on the expression of transient receptor potential va-nilloid subfamily 1(TRPV1),substance P(SP),and calcitonin gene-related peptide(CGRP)in the dorsal root ganglion(DRG)of the guinea pig model of cough hypersensitivity,and decipher the mechanism of Qufeng Xuanfei Decoction in relieving chronic cough.Methods:Forty-eight SPF-grade healthy male guinea pigs were randomized into normal control(normal saline),model(nor-mal saline),Western medicine(capsazepine),and Chinese medicine(Qufeng Xuanfei Decoction)groups.The guinea pig model of cough hypersensitivity was established by sensitization with ovalbumin.After one week of drug intervention,the cough times of guinea pigs in each group were measured by capsaicin atomization.The mRNA levels of TRPV1,SP,and CGRP in the DRG were measured by RT-PCR,and the protein levels of TRPV1,NK-1 receptor(NK1-R),and CGRP in the DRG were measured by West-ern blotting.Results:Compared with the normal control group,the model group showed increased cough times,up-regulated mRNA levels of TRPV1,SP,and CGRP and up-regulated protein levels of TRPV1,NK-1R,and CGRP in the DRG(all P＜0.01).Com-pared with the model group,both Western medicine and Chinese medicine reduced the cough times(P＜0.05)and down-regulated the mRNA levels of TRPV1,SP,and CGRP in the DRG(P＜0.01).In addition,the Western medicine down-regulated the protein levels of TRPV1,NK-1R and CGRP in the DRG(P＜0.05)and the Chinese medicine down-regulated the protein levels of NK-1R and CGRP(P＜0.05).The protein level of TRPV1 showed no significant difference between the Chinese medicine group and the model group(P＞0.05).Conclusion:Qufeng Xuanfei Decoction may block the TRPV1 channel and inhibit the dorsal root reflex to reduce the neurogenic inflammation in the airway.关键词
祛风宣肺方/慢性咳嗽/咳嗽敏感性增高/瞬时受体电位香草酸亚型1/背根神经节/背根神经反射/气道神经源性炎症Key words
Qufeng Xuanfei Decoction/Chronic cough/Cough hypersensitivity/Transient receptor potential vanilloid 1/Dorsal root ganglion/Dorsal root reflex/Neurogenic inflammation in the airway分类
医药卫生引用本文复制引用
李雅兰,马建岭,史利卿,王颖,罗敬月,罗景舒,李扭扭..祛风宣肺方通过背根神经反射降低咳嗽敏感性的作用机制研究[J].世界中医药,2023,18(21):3060-3064,5.基金项目
国家自然科学基金面上项目(81373588) （81373588）
北京市自然科学基金项目(7192115,7184224) （7192115,7184224）
北京中医药大学东方医院"1166"人才培养项目(040204001001002012) （040204001001002012）
中国民族医药学会科研项目(2021Z1215-510201) （2021Z1215-510201）
