饲料研究2023，Vol.46Issue(23)：96-101,6.DOI:10.13557/j.cnki.issn1002-2813.2023.23.018
超声波-酶协同提取女贞子总黄酮工艺及其抗氧化活性研究
Study on ultrasonic and enzyme synergistic extraction of total flavonoids from Fructus ligustris L.and its antioxidant activity
摘要
Abstract
On the basis of single factor test,response surface method was used to optimize the extraction process of Fructus ligustris L.,and the in vitro antioxidant activity of total flavonoids from Fructus ligustris L.was studied.The results showed that the optimal process parameters were ethanol concentration of 80%,cellulase concentration of 0.3 g/L and ultrasonic temperature of 50℃.Under these conditions,the average extraction rate of total flavonoids from Fructus ligustris L.was 4.93%,which was highly consistent with the predicted value of 4.98%,indicating that the model was reliable and could be used for the extraction of total flavonoids from Fructus ligustris L..The results of antioxidant test showed that the IC50 value of total flavonoids extract was 186.6 mg/L for DPPH free radical scavenging and 166.8 mg/L for ABTS+ free radical scavenging,indicating that total flavonoids extract had strong antioxidant activity.The study indicates that total flavonoids of Fructus ligustris L.can be used as a natural green antioxidant in animal production.关键词
女贞子/纤维素酶/超声波/黄酮/抗氧化活性Key words
Fructus ligustris L./cellulase/ultrasonic wave/flavonoids/antioxidant activity分类
农业科技引用本文复制引用
王令智,陈平,郭振华,张洪磊..超声波-酶协同提取女贞子总黄酮工艺及其抗氧化活性研究[J].饲料研究,2023,46(23):96-101,6.基金项目
湖南省自然科学基金项目(项目编号:2021HJ30552) （项目编号:2021HJ30552）
湖南省农业科技创新资金项目(项目编号:2022CX66) （项目编号:2022CX66）
