实验动物与比较医学2023，Vol.43Issue(6)：612-618,7.DOI:10.12300/j.issn.1674-5817.2023.035
Big-BALB/c小鼠亚系选育前后的抗体制备效率比较研究
Study on the Antibody Production Efficiency in Modified Big-BALB/c Mice
摘要
Abstract
Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c(B-BALB/c)mice,and to provide a theoretical basis and reference for the selection of laboratory animals in the preparation of monoclonal antibodies induced in vivo through hybridoma.Methods Individuals weighing more than 5%of the weight of normal animals at 4 weeks of age(the criterion for late selection is more than 10%)were selected from a population of conventionally bred BALB/c mice and bred individually,and a subline of B-BALB/c mice was prepared after 10 generations of selection.A total of 40 BALB/c mice and 40 B-BALB/c mice aged 10 to 11 weeks,half male and half female,were selected and inoculated with the mousepox monoclonal antibody hybridoma cell line G23 or the murine hepatitis monoclonal antibody hybridoma cell line Y15 pre-treated with liquid paraffin,respectively.Mice ascites containing monoclonal antibodies were obtained by in vivo induction.The antibody titer was tested by indirect ELISA.The mice were grouped based on the sub-strains,gender and inoculation type of hybridoma to analyze the ascites production,antibody titer and antibody production,and to evaluate the antibody preparation efficiency of the two BALB/c mouse sub-strains.Results After 10 generations of breeding,the body weight of 10-week-old male and female B-BALB/c mice increased by 22.3%and 12.8%,respectively,compared with BALB/c mice of the same age.Compared with BALB/c mice,B-BALB/c mice had better tolerance and adaptation to secondary ascites collection.Compared with BALB/c mice,the ascites production and antibody titer during the preparation of antibodies in B-BALB/c mice were significantly increased,especially in the hybridoma cell line G23 vaccination group(both P＜0.000 1).After inoculation with the hybridoma cell lines G23 or Y15,the average antibody production of B-BALB/c mice(14.99×104 U and 33.22×104 U)was higher than that of BALB/c mice(5.33×104 U and 19.31×104 U)(both P＜0.01).After inoculation with hybridoma cell line G23,the average antibody production per unit body weight of B-BALB/c mice(0.55×104 U/g)was higher than that of BALB/c mice(0.23×104 U/g)(P＜0.000 1).And the antibody production per unit body weight of female B-BALB/c or BALB/c mice was higher than that of male B-BALB/c or BALB/c mice(both P＜0.01).Conclusion B-BALB/c mice can be used as an alternative to BALB/c mice in the in vivo induction of monoclonal antibody preparation,which can achieve the purpose of reducing the number of experimental animals used,lowering the labor cost,and improving the efficiency of antibody preparation.关键词
单克隆抗体/制备效率/杂交瘤/BALB/c小鼠/Big-BALB/c小鼠Key words
Monocloning antibody/Production efficiency/Hybridomas/BALB/c mice/Big-BALB/c mice分类
医药卫生引用本文复制引用
王丹,杜小燕,战大伟,章秀林,李长龙,张晓璐,王妍,傅博,王文栋,刘京,张甦寅,武怡荷,吴德国..Big-BALB/c小鼠亚系选育前后的抗体制备效率比较研究[J].实验动物与比较医学,2023,43(6):612-618,7.基金项目
国家重点研发计划项目课题"基因编辑、SPF级和无菌级实验动物质量评价技术标准研究"(2022YFF0711005) （2022YFF0711005）
国家重点研发计划青年科学家项目"基于SNP技术建立我国常用实验动物遗传质量评价技术体系"(2021YFF0703200) （2021YFF0703200）
国家自然科学基金"长爪沙鼠长期感染的幽门螺杆菌遗传变异及对胃内微生物种群和病理变化的影响"(32070537) （32070537）
北京市自然科学基金-北京市教育委员会重点项目"幽门螺杆菌调节RXR诱发长爪沙鼠胃癌的机制研究"(KZ20210025037) （KZ20210025037）
