陕西医学杂志2024，Vol.53Issue(1)：122-126,5.DOI:10.3969/j.issn.1000-7377.2024.01.027
丝裂原活化蛋白激酶4、生存素基因在喉鳞状细胞癌组织中的表达及其意义
Expression and significance of MKK4 and Survivin genes in laryngeal squamous cell carcinoma
摘要
Abstract
Objective:To investigate the expression and significance of mitogen-activated protein kinase kinase 4(MKK4)and Survivin in laryngeal squamous cell carcinoma.Methods:Eighty-two cases of laryngeal cancer were se-lected as the research objects.The expression levels of MKK4 and Survivin in laryngeal cancer tissues and adjacent normal mucosa tissues were detected by RT-PCR and immunohistochemistry,and the relationship between MKK4,Survivin and clinicopathological characteristics was analyzed.The patients were followed up for 5 years,and the sur-vival rate of patients with laryngeal cancer was recorded.Cox proportional hazards regression model was used to ana-lyze the prognostic factors of patients with laryngeal cancer.Results:The expression of MKK4 mRNA in laryngeal carcinoma tissues was lower than that in paracancer tissues,while Survivin mRNA expression was higher in laryngeal carcinoma tissues(all P＜0.05).The positive expression rates of MKK4 protein in adjacent tissues and laryngeal cancer tissues were 71.95％(59/82)and 40.24％(33/82),and the positive expression rates of Survivin protein were 23.17％(19/82)and 71.95％(59/82),respectively(x2=16.737,39.117,all P＜0.01).There were significant differ-ences in the expression of MKK4 and Survivin in tumor differentiation,TNM stage,and lymph node metastasis in la-ryngeal carcinoma tissues(all P＜0.05).There was a negative correlation between MKK4 and Survivin expression in laryngeal carcinoma tissues(r=-0.403,P＜0.05).The 5-year survival rate of MKK4-positive patients was 81.82％(27/33),higher than 65.31％(32/49)of negative patients(P＜0.05).The 5-year survival rate of survivin-positive patients was 62.71％(37/59),lower than 82.61％(19/23)of negative patients(P＜0.05).TNM stage,MKK4 and Survivin were independent prognostic factors in patients with laryngeal cancer(all P＜0.05).Conclusion:MKK4 ex-pression is down-regulated and Survivin expression is up-regulated in laryngeal carcinoma tissues,which may be po-tential prognostic markers and therapeutic targets.关键词
丝裂原活化蛋白激酶4/生存素/喉鳞状细胞癌/标记物/预后Key words
Mitogen-activated protein kinase kinase 4/Survivin/Laryngeal squamous cell carcinoma/Marker/Prognosis分类
医药卫生引用本文复制引用
单凤金,张义,许尧生..丝裂原活化蛋白激酶4、生存素基因在喉鳞状细胞癌组织中的表达及其意义[J].陕西医学杂志,2024,53(1):122-126,5.基金项目
河北省医学科学研究计划课题(20210799) （20210799）
