药学研究2023，Vol.42Issue(12)：953-958,6.DOI:10.13506/j.cnki.jpr.2023.12.001
鱼鳞膏调节IL-6/STAT3信号通路对难愈性皮肤溃疡模型大鼠创面愈合的影响
Impact of Fish Scale Ointment on wound healing in rats with refractory skin ulcers by regulating the IL-6/STAT3 signaling pathway
摘要
Abstract
Objective To explore the impact of Fish Scale Ointment on wound healing in rats with refractory skin ulcer by regulating interleukin-6(IL-6)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods The model of diabetes rats was established by feeding with high fat and high sugar and intraperitoneal injection of strepto-zotocin(STZ),and the model of refractory skin ulcer was established by removing the back skin through surgical scissors;After successful modeling,the rats were randomly divided into model group,Fish Scale Ointment group and activator group according to the random number table method,with 12 rats in each group.Another 12 rats were taken as the control group(model control),and the corresponding drugs were applied and injected intraperitoneally in each group,once a day for 21 days;The wound healing status of rats was measured after administration;HE staining was applied to observe wound damage and neovascularization;Enzyme linked immunosorbent assay(ELISA)was applied to detect the levels of interleukin-6,-1β(IL-6,IL-1β)and tumor necrosis factor-α(TNF-α)in placental wound tissue;Immunohistochemical staining was ap-plied to determine the expression of basic fibroblast growth factor(bFGF)and platelet endothelial cell adhesion molecule 1(CD31);Real-time fluorescence quantitative PCR(qRT-PCR)method was applied to detect the expression of IL-6 and STAT3 mRNA in wound tissue;And Western blotting was applied to detect the expression of vascular endothelial growth fac-tor(VEGF),transforming growth factor-β1(TGF-β1)and IL-6/STAT3 pathway related proteins in rat wound tissues.Results Compared with the control group,the wound healing rate,number of new blood vessels,and the expression levels of bFGF,CD31,VEGF,and TGF-β1 proteins in the model group were obviously reduced,the levels of IL-6,IL-1β,TNF-α,and the expression levels of IL-6,STAT3 mRNAs and proteins were obviously increased(P<0.05).Compared with the model group,the wound healing rate,number of new blood vessels,and the expression levels of bFGF,CD31,VEGF,and TGF-β1 proteins in the Fish Scale Ointment group were obviously increased,the levels of IL-6,IL-1β,TNF-α,and the expression levels of IL-6,STAT3 mRNAs and proteins were obviously reduced(P<0.05);Compared with the Fish Scale Ointment group,the changes in the above indicators in the activator group were obviously reversed(P<0.05).Conclusion Fish Scale Ointment has the effects of inhibiting inflammatory response,promoting angiogenesis and wound healing in rats with refractory skin ulcers,and its mechanism of action may be related to the inhibition of IL-6/STAT3 signaling pathway.关键词
鱼鳞膏/白细胞介素-6/信号转导与转录激活因子3/难愈性皮肤溃疡/创面愈合/炎症反应Key words
Fish Scale Ointment/Interleukin-6/Signal transducer and activator of transcription 3/Refractory skin ul-cer/Wound healing/Inflammatory response分类
中医学引用本文复制引用
王晋军,王品一,黄贤明,张中旺,陈啸,李强..鱼鳞膏调节IL-6/STAT3信号通路对难愈性皮肤溃疡模型大鼠创面愈合的影响[J].药学研究,2023,42(12):953-958,6.基金项目
国家自然科学基金面上项目(No.82172095) （No.82172095）
青岛市中医药科技项目(No.2022-zyym03) （No.2022-zyym03）
