药学研究2023，Vol.42Issue(12)：964-968,977,6.DOI:10.13506/j.cnki.jpr.2023.12.003
夏桑菊浸膏对高渗诱导干眼模型人角膜上皮细胞保护机制研究
Protective mechanism of different Xiasangju extract on corneal epithelial cells in a dry eye model induced by hypertonic fluid
李进良 1胡双飞 1李沛波 1吴灏 1彭维 1苏薇薇 1王永刚 1孙维广2
作者信息
- 1. 中山大学生命科学学院,广东省中药上市后质量与药效再评价工程技术研究中心/广东省热带亚热带植物资源重点实验室,广东 广州 510275
- 2. 广州白云山星群<药业>股份有限公司,广东 广州 510288
- 折叠
摘要
Abstract
Objective To investigate the protective effect and mechanism of the extract of Xiasangju on human corneal epithelial cells induced by hypertonic.Methods Hypertonic model was constructed with NaCl,and cell viability was detec-ted by MTS method.The expressions of IL-1β,IL-6 and IL-18 were detected by RT-qPCR and enzyme-linked immu-nosorbent assay.Intracellular reactive oxygen species(ROS)were detected by 2',7'-dichlorofluorescein yellow diacetate fluorescence probe.Results Compared with the control group,the cell vitality of the model group at 600 mOsm·L-1 was sig-nificantly decreased,and the cell vitality of the model group was significantly increased after treatment with the extract of Xi-asangju.In terms of inflammatory indicators,the secretion levels of IL-1β,IL-6 and IL-18 and the expression levels of IL-1β in 450 mOsm·L-1 cells in the model group were significantly increased compared with the control group.Compared with 450 mOsm·L-1 model group,the above indexes were significantly decreased after treatment with the extract of Xiasangju.In the antioxidant study,the reactive oxygen species in 450 mOsm·L-1 cells in the model group was significantly increased,while the reactive oxygen species was significantly decreased after treatment with Xiasangju extract.Conclusion The extract of Xiasangju can inhibit the decrease of human corneal epithelial cell vitality induced by hypertonic,and can protect human corneal epithelial cells in hypertonic environment by inhibiting the production of inflammatory factors and inhibiting the increase of reactive oxygen species.关键词
夏桑菊浸膏/人角膜上皮细胞/高渗/干眼症Key words
Extract of Xiasangju/Human corneal epithelial cells/Hyperosmotic stress/Dry eye disease分类
医药卫生引用本文复制引用
李进良,胡双飞,李沛波,吴灏,彭维,苏薇薇,王永刚,孙维广..夏桑菊浸膏对高渗诱导干眼模型人角膜上皮细胞保护机制研究[J].药学研究,2023,42(12):964-968,977,6.
